U.S. Fish and Wildlife Service, Lakewood, Colorado, USA.
Environ Toxicol Chem. 2011 Feb;30(2):274-82. doi: 10.1002/etc.402.
Over the last decade, the environmental sciences have witnessed an incredible movement towards the utilization of high-throughput molecular tools that are capable of detecting simultaneous changes of hundreds, and even thousands, of molecules and molecular components after exposure of organisms to different environmental stressors. These techniques have received a great deal of attention because they not only offer the potential to unravel novel mechanisms of physiological and toxic action but are also amenable to the discovery of biomarkers of exposure and effects. In this article, we review the state of knowledge of one of these tools in ecotoxicological research: proteomics. We summarize the state of proteomics research in fish, and follow with studies conducted with aquatic invertebrates. A brief discussion on proteomic methods is also presented. We conclude with some ideas for future proteomic studies with fish and aquatic invertebrates.
在过去的十年中，环境科学见证了一场令人难以置信的运动，即利用高通量分子工具来检测生物体暴露于不同环境胁迫后数百甚至数千种分子和分子成分的同时变化。这些技术引起了广泛关注，因为它们不仅有可能揭示生理和毒性作用的新机制，而且还适用于发现暴露和效应的生物标志物。在本文中，我们回顾了生态毒理学研究中其中一种工具的知识状态：蛋白质组学。我们总结了鱼类蛋白质组学研究的现状，并接着介绍了水生无脊椎动物的研究。还简要讨论了蛋白质组学方法。最后，我们提出了一些关于鱼类和水生无脊椎动物未来蛋白质组学研究的想法。
