小菜蛾两种化感蛋白基因的克隆、表达谱分析及 5'调控区序列分析

Molecular cloning, expression profile and 5' regulatory region analysis of two chemosensory protein genes from the diamondback moth, Plutella xylostella.

机构信息

Key Laboratory of Pesticide and Chemical Biology, Ministry of Education of P.R. China. South China Agricultural University, Guang Zhou 510642, Guangdong, China.

出版信息

J Insect Sci. 2010;10:143. doi: 10.1673/031.010.14103.

DOI:10.1673/031.010.14103

PMID:21073345

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3016857/

Abstract

Chemosensory proteins play an important role in transporting chemical compounds to their receptors on dendrite membranes. In this study, two full-length cDNA codings for chemosensory proteins of Plutella xylostella (Lepidoptera: Plutellidae) were obtained by RACE-PCR. PxylCSP3 and Pxyl-CSP4, with GenBank accession numbers ABM92663 and ABM92664, respectively, were cloned and sequenced. The gene sequences both consisted of three exons and two introns. RT-PCR analysis showed that Pxyl-CSP3 and Pxyl-CSP4 had different expression patterns in the examined developmental stages, but were expressed in all larval stages. Phylogenetic analysis indicated that lepidopteran insects consist of three branches, and Pxyl-CSP3 and Pxyl-CSP4 belong to different branches. The 5'regulatory regions of Pxyl-CSP3 and Pxyl-CSP4 were isolated and analyzed, and the results consist of not only the core promoter sequences (TATA-box), but also several transcriptional elements (BR-C Z4, Hb, Dfd, CF2-II, etc.). This study provides clues to better understanding the various physiological functions of CSPs in P. xylostella and other insects.

摘要

化学感觉蛋白在将化合物运输到树突膜上的受体中起着重要作用。在这项研究中，通过 RACE-PCR 获得了小菜蛾（鳞翅目：菜蛾科）的两种全长化学感觉蛋白 cDNA 编码。PxylCSP3 和 Pxyl-CSP4，GenBank 登录号分别为 ABM92663 和 ABM92664，被克隆和测序。基因序列均由三个外显子和两个内含子组成。RT-PCR 分析表明，Pxyl-CSP3 和 Pxyl-CSP4 在检查的发育阶段具有不同的表达模式，但在所有幼虫阶段都有表达。系统发育分析表明，鳞翅目昆虫分为三个分支，Pxyl-CSP3 和 Pxyl-CSP4 属于不同的分支。分离并分析了 Pxyl-CSP3 和 Pxyl-CSP4 的 5'调控区，结果不仅包含核心启动子序列（TATA 盒），还包含几个转录因子（BR-C Z4、Hb、Dfd、CF2-II 等）。本研究为更好地理解 CSP 在小菜蛾和其他昆虫中的各种生理功能提供了线索。