Key Laboratory of Pesticide and Chemical Biology, Ministry of Education of P.R. China. South China Agricultural University, Guang Zhou 510642, Guangdong, China.
J Insect Sci. 2010;10:143. doi: 10.1673/031.010.14103.
Chemosensory proteins play an important role in transporting chemical compounds to their receptors on dendrite membranes. In this study, two full-length cDNA codings for chemosensory proteins of Plutella xylostella (Lepidoptera: Plutellidae) were obtained by RACE-PCR. PxylCSP3 and Pxyl-CSP4, with GenBank accession numbers ABM92663 and ABM92664, respectively, were cloned and sequenced. The gene sequences both consisted of three exons and two introns. RT-PCR analysis showed that Pxyl-CSP3 and Pxyl-CSP4 had different expression patterns in the examined developmental stages, but were expressed in all larval stages. Phylogenetic analysis indicated that lepidopteran insects consist of three branches, and Pxyl-CSP3 and Pxyl-CSP4 belong to different branches. The 5'regulatory regions of Pxyl-CSP3 and Pxyl-CSP4 were isolated and analyzed, and the results consist of not only the core promoter sequences (TATA-box), but also several transcriptional elements (BR-C Z4, Hb, Dfd, CF2-II, etc.). This study provides clues to better understanding the various physiological functions of CSPs in P. xylostella and other insects.
化学感觉蛋白在将化合物运输到树突膜上的受体中起着重要作用。在这项研究中，通过 RACE-PCR 获得了小菜蛾（鳞翅目：菜蛾科）的两种全长化学感觉蛋白 cDNA 编码。PxylCSP3 和 Pxyl-CSP4，GenBank 登录号分别为 ABM92663 和 ABM92664，被克隆和测序。基因序列均由三个外显子和两个内含子组成。RT-PCR 分析表明，Pxyl-CSP3 和 Pxyl-CSP4 在检查的发育阶段具有不同的表达模式，但在所有幼虫阶段都有表达。系统发育分析表明，鳞翅目昆虫分为三个分支，Pxyl-CSP3 和 Pxyl-CSP4 属于不同的分支。分离并分析了 Pxyl-CSP3 和 Pxyl-CSP4 的 5'调控区，结果不仅包含核心启动子序列（TATA 盒），还包含几个转录因子（BR-C Z4、Hb、Dfd、CF2-II 等）。本研究为更好地理解 CSP 在小菜蛾和其他昆虫中的各种生理功能提供了线索。
