Rodríguez-Rodríguez L, García-Bermúdez M, González-Juanatey C, Vazquez-Rodriguez T R, Miranda-Filloy J A, Fernandez-Gutierrez B, Llorca J, Martin J, González-Gay M A
Instituto de Parasitología y Biomedicina López-Neyra, C.S.I.C., Granada, Spain.
Tissue Antigens. 2011 Jan;77(1):74-8. doi: 10.1111/j.1399-0039.2010.01580.x. Epub 2010 Nov 14.
To assess the potential association between ADIPOQ rs266729 and rs1501299 gene polymorphisms, either isolated or in combination, and cardiovascular disease in patients with rheumatoid arthritis (RA), 674 patients seen at the rheumatology outpatient clinics of Hospital Xeral-Calde, Lugo, and Hospital San Carlos, Madrid, Spain, were analyzed. Genotyping was performed using predesigned TaqMan assays (Applied Biosystems, Foster City, CA). Carotid intima-media thickness, flow-mediated endothelium-dependent and endothelium-independent post-nitroglycerin vasodilatation, which are used as surrogate markers of subclinical atherosclerosis, were measured in a subsample. No significant differences in the genotype, allele or allele combination frequencies of both polymorphisms were found between RA patients with or without cardiovascular events or subclinical atherosclerosis. Therefore, ADIPOQ rs266729 and rs1501299 polymorphisms do not seem to be associated with cardiovascular disease in RA.
为评估脂联素基因（ADIPOQ）rs266729和rs1501299基因多态性单独或联合存在时与类风湿关节炎（RA）患者心血管疾病之间的潜在关联，对西班牙卢戈市塞拉尔 - 卡尔德医院和马德里圣卡洛斯医院风湿病门诊的674例患者进行了分析。使用预先设计的TaqMan检测法（应用生物系统公司，加利福尼亚州福斯特城）进行基因分型。在一个子样本中测量了颈动脉内膜中层厚度、血流介导的内皮依赖性和硝酸甘油后内皮非依赖性血管舒张，这些指标被用作亚临床动脉粥样硬化的替代标志物。在有或没有心血管事件或亚临床动脉粥样硬化的RA患者之间，未发现这两种多态性的基因型、等位基因或等位基因组合频率存在显著差异。因此，ADIPOQ rs266729和rs1501299多态性似乎与RA患者的心血管疾病无关。
