检测克罗恩病患儿肠道活检中的螺旋杆菌科。
Detection of Helicobacteraceae in intestinal biopsies of children with Crohn's disease.
机构信息
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
出版信息
Helicobacter. 2010 Dec;15(6):549-57. doi: 10.1111/j.1523-5378.2010.00792.x.
BACKGROUND
Given that members of Helicobacteraceae family colonize the intestinal mucus layer, it has been hypothesized that they may play a role in Crohn's disease. This study investigated the presence of Helicobacteraceae DNA in biopsies collected from children with Crohn's disease and controls.
MATERIALS AND METHODS
The presence of Helicobacteraceae DNA was investigated in intestinal biopsies collected from 179 children undergoing colonoscopy (Crohn's disease n = 77, controls n = 102) using a Helicobacteraceae-specific PCR.
RESULTS
Members of the Helicobacteraceae were detected in 32/77 children with Crohn's disease (41.5%) and 23/102 controls (22.5%). Statistical analysis showed the prevalence of Helicobacteraceae detected in patients to be significantly higher than that in controls (p = .0062). Analysis of non-pylori Helicobacteraceae showed that their prevalence was also significantly higher in patients than in controls (p = .04). Helicobacter pylori was detected in 14.0% of the biopsies across all groups. Given that all children tested were negative for gastric H. pylori, this was a surprising finding. Phylogenetic analysis of H. pylori sequences detected in the biopsies showed that the H. pylori strains identified in the patients did not group with gastric H. pylori included in the analysis, but rather with other H. pylori strains detected in the intestine, gall bladder, and liver.
CONCLUSIONS
The higher prevalence of Helicobacteraceae DNA in Crohn's disease patients would suggest that members of this family may be involved in this disease. In addition, phylogenetic analysis of H. pylori strains showed that extragastric sequences clustered together, indicating that different H. pylori strains may adapt to colonize extragastric niches.
背景
鉴于幽门螺旋杆菌科的成员定植于肠道黏液层，因此推测它们可能在克罗恩病中发挥作用。本研究旨在调查克罗恩病患儿和对照者的肠道活检组织中是否存在幽门螺旋杆菌科的 DNA。
材料和方法
采用幽门螺旋杆菌科特异性 PCR 法，检测 179 例行结肠镜检查的儿童（克罗恩病患者 n=77，对照者 n=102）的肠道活检组织中幽门螺旋杆菌科的 DNA。
结果
在 77 例克罗恩病患儿中，32 例（41.5%）和 102 例对照者中 23 例（22.5%）检测到幽门螺旋杆菌科成员。统计学分析显示，患者中幽门螺旋杆菌科的检出率明显高于对照组（p=0.0062）。非幽门螺旋杆菌科幽门螺旋杆菌的分析显示，其在患者中的检出率也明显高于对照组（p=0.04）。所有患儿的胃 H. pylori 检测均为阴性，而在所有活检组织中均检测到 H. pylori 14.0%。这一发现令人惊讶。对活检组织中检测到的 H. pylori 序列进行系统发育分析显示，患者中鉴定的 H. pylori 菌株与分析中包含的胃 H. pylori 菌株不同，而是与肠道、胆囊和肝脏中检测到的其他 H. pylori 菌株聚在一起。
结论
克罗恩病患者中幽门螺旋杆菌科 DNA 的更高检出率提示该家族成员可能与该病有关。此外，H. pylori 菌株的系统发育分析显示，胃外序列聚类在一起，表明不同的 H. pylori 菌株可能适应定植于胃外生态位。
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