Riva Federica, Bianchessi Laura, Recordati Camilla, Inglesi Alessia, Castiglioni Vittoria, Turin Lauretta
Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell'Università 6, 26900 Lodi, Italy.
Idexx Laboratories Italia, Via Guglielmo Silva 36, 20149 Milan, Italy.
Vet Sci. 2022 Jun 14;9(6):295. doi: 10.3390/vetsci9060295.
Chronic enteropathy (CE) is a severe multifactorial gastrointestinal disease that affects dogs and is driven by poorly characterized inflammatory pathways. Imbalance of pro-inflammatory response regulators, including IL-1R8, may be due to different factors, among which the infection with is known to lead to a vicious circle in which excessive pro-inflammatory signaling and gastrointestinal injury reinforce each other and boost the disease. We investigated the expression of IL-1R8 in large intestine biopsies of dogs with or without clinical signs of CE and with previously assessed enterohepatic spp. colonization status by mean of quantitative real-time PCR. Our study revealed that IL-1R8 is downregulated in both acutely ( = 0.0074) and chronically ( = 0.0159) CE affected dogs compared to healthy controls. The data also showed that IL-1R8 expression tends to decrease with colonization by spp. Interestingly, a negative correlation was detected between the level of expression of IL-1R8 and the severity of macroscopic lesions identified by endoscopy and the crypt hyperplasia score. We further compared the expression levels between males and females and found no statistically significant difference between the two groups. No significant difference was observed in IL-1R8 expression profiles with the age of the animals either. Interestingly, an association was uncovered between IL-1R8 expression level and dog breed. Together, our data advance knowledge on gastrointestinal pathoimmunology in dogs and highlight the potential utilization of IL-1R8 as a diagnostic, prognostic and therapeutic biomarker for canine chronic enteropathy.
慢性肠病（CE）是一种严重的多因素胃肠道疾病，影响犬类，由特征不明的炎症途径驱动。促炎反应调节因子失衡，包括白细胞介素-1受体8（IL-1R8），可能由不同因素引起，其中已知感染[具体病原体未给出]会导致恶性循环，即过度的促炎信号和胃肠道损伤相互强化，加剧疾病。我们通过定量实时聚合酶链反应，研究了有无CE临床症状以及先前评估过肝肠[具体病原体未给出]定植状态的犬类大肠活检组织中IL-1R8的表达。我们的研究表明，与健康对照相比，急性（P = 0.0074）和慢性（P = 0.0159）CE感染犬的IL-1R8均下调。数据还显示，IL-1R8表达倾向于随着[具体病原体未给出]的定植而降低。有趣的是，检测到IL-1R8表达水平与内镜检查确定的宏观病变严重程度和隐窝增生评分之间存在负相关。我们进一步比较了雄性和雌性之间的表达水平，发现两组之间无统计学显著差异。在不同年龄动物的IL-1R8表达谱中也未观察到显著差异。有趣的是，发现IL-1R8表达水平与犬种之间存在关联。总之，我们的数据推进了对犬类胃肠道病理免疫学的认识，并突出了IL-1R8作为犬慢性肠病诊断、预后和治疗生物标志物的潜在用途。
