State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210093, PR China.
Environ Int. 2012 Jul;42:117-23. doi: 10.1016/j.envint.2011.05.013. Epub 2011 Jun 17.
Thyroid hormone disrupting compounds in water sources is a concern. Thyroid hormone (TH) agonist and antagonist activities of water sources from the Yangtze River, Huaihe River, Taihu Lake and ground water in the Yangtze River Delta region were evaluated by use of a TH reporter gene assay based on the green monkey kidney fibroblast (CV-1). While weak TH receptor (TR) agonist potency was observed in only one of 15 water sources, antagonist potency was present in most of the water sources. TR antagonist equivalents could be explained by the presence of dibutyl phthalate (DBP), with concentrations ranging from 2.8×10(1) to 1.6×10(3) μg DBP /L (ATR-EQ(50)s). None of the ground waters exhibited TH agonist potencies while all of the samples from Taihu Lake displayed notable TR antagonist potencies. To identify the responsible thyroid active compounds, instrumental analysis was conducted to measure a list of potential thyroid-disrupting chemicals, including organochlorine (OC) pesticides and phthalate esters. Combining the results of the instrumental analysis with those of the bioassay, DBP was determined to account for 17% to 144% of ATR-EQ(50)s in water sources. Furthermore, ATR-EQ(20-80) ranges for TR antagonist activities indicated that samples from locations WX-1 and WX-2 posed the greatest health concern and the associated uncertainty may warrant further investigation.
水源中的甲状腺激素干扰化合物令人担忧。本研究采用基于绿猴肾成纤维细胞（CV-1）的甲状腺激素报告基因检测法，评估了长江、淮河、太湖及长三角地区地下水等水源中的甲状腺激素（TH）激动剂和拮抗剂活性。在 15 种水源中，仅有 1 种表现出较弱的 TH 受体（TR）激动剂活性，而大多数水源均具有 TR 拮抗剂活性。二丁基邻苯二甲酸酯（DBP）的存在可以解释这种 TR 拮抗剂活性，其浓度范围为 2.8×10(1)至 1.6×10(3)μg DBP/L（ATR-EQ(50)s）。所有地下水均未表现出 TH 激动剂活性，而太湖水样均表现出显著的 TR 拮抗剂活性。为了鉴定具有甲状腺活性的化合物，本研究采用仪器分析方法对一系列潜在的甲状腺干扰化学物质进行了测量，包括有机氯农药和邻苯二甲酸酯。将仪器分析结果与生物测定结果相结合，发现 DBP 占水源中 ATR-EQ(50)s 的 17%至 144%。此外，TR 拮抗剂活性的 ATR-EQ(20-80)范围表明，WX-1 和 WX-2 两个地点的水样具有最大的健康风险，相关不确定性可能需要进一步调查。
