登革病毒诱导的自噬调节脂质代谢。
Dengue virus-induced autophagy regulates lipid metabolism.
机构信息
Department of Microbiology, The University of Chicago, Chicago, IL 60637, USA.
出版信息
Cell Host Microbe. 2010 Nov 18;8(5):422-32. doi: 10.1016/j.chom.2010.10.006.
Abstract
Autophagy influences numerous cellular processes, including innate and adaptive immunity against intracellular pathogens. However, some viruses, including dengue virus (DENV), usurp autophagy to enhance their replication. The mechanism for a positive role of autophagy in DENV infection is unclear. We present data that DENV induction of autophagy regulates cellular lipid metabolism. DENV infection leads to an autophagy-dependent processing of lipid droplets and triglycerides to release free fatty acids. This results in an increase in cellular β-oxidation, which generates ATP. These processes are required for efficient DENV replication. Importantly, exogenous fatty acids can supplant the requirement of autophagy in DENV replication. These results define a role for autophagy in DENV infection and provide a mechanism by which viruses can alter cellular lipid metabolism to promote their replication.
摘要
自噬影响众多细胞过程，包括固有和适应性免疫应答以抵抗细胞内病原体。然而，一些病毒，包括登革热病毒（DENV），利用自噬来增强其复制。自噬在 DENV 感染中发挥积极作用的机制尚不清楚。我们提供的数据表明，DENV 诱导的自噬调节细胞脂质代谢。DENV 感染导致脂滴和甘油三酯的自噬依赖性加工，以释放游离脂肪酸。这导致细胞β-氧化增加，从而产生 ATP。这些过程对于 DENV 的有效复制是必需的。重要的是，外源性脂肪酸可以替代自噬在 DENV 复制中的需求。这些结果定义了自噬在 DENV 感染中的作用，并提供了一种机制，病毒可以通过改变细胞脂质代谢来促进其复制。
相似文献
1
Dengue virus-induced autophagy regulates lipid metabolism.登革病毒诱导的自噬调节脂质代谢。
Cell Host Microbe. 2010 Nov 18;8(5):422-32. doi: 10.1016/j.chom.2010.10.006.
2
Dengue virus and autophagy.登革热病毒与自噬。
Viruses. 2011 Aug;3(8):1332-41. doi: 10.3390/v3081332. Epub 2011 Aug 4.
3
Dengue Virus Activates the AMP Kinase-mTOR Axis To Stimulate a Proviral Lipophagy.登革病毒激活AMP激酶-mTOR轴以刺激一种前病毒自噬。
J Virol. 2017 May 12;91(11). doi: 10.1128/JVI.02020-16. Print 2017 Jun 1.
4
Exploitation of cellular pathways by Dengue virus.登革病毒对细胞通路的利用。
Curr Opin Microbiol. 2011 Aug;14(4):470-5. doi: 10.1016/j.mib.2011.07.012. Epub 2011 Jul 27.
5
Reduced sphingosine kinase 1 activity in dengue virus type-2 infected cells can be mediated by the 3' untranslated region of dengue virus type-2 RNA.感染登革病毒 2 型的细胞中鞘氨醇激酶 1 活性降低可由登革病毒 2 型 RNA 的 3'非翻译区介导。
J Gen Virol. 2013 Nov;94(Pt 11):2437-2448. doi: 10.1099/vir.0.055616-0. Epub 2013 Aug 12.
6
Induced autophagy reduces virus output in dengue infected monocytic cells.诱导自噬可降低登革热感染单核细胞中的病毒产量。
Virology. 2011 Sep 15;418(1):74-84. doi: 10.1016/j.virol.2011.07.010. Epub 2011 Aug 2.
7
Dengue Virus Inhibition of Autophagic Flux and Dependency of Viral Replication on Proteasomal Degradation of the Autophagy Receptor p62.登革病毒对自噬流的抑制作用以及病毒复制对自噬受体p62蛋白酶体降解的依赖性。
J Virol. 2015 Aug;89(15):8026-41. doi: 10.1128/JVI.00787-15.
8
Rab18 facilitates dengue virus infection by targeting fatty acid synthase to sites of viral replication.Rab18 通过将脂肪酸合酶靶向病毒复制部位促进登革病毒感染。
J Virol. 2014 Jun;88(12):6793-804. doi: 10.1128/JVI.00045-14. Epub 2014 Apr 2.
9
A role for autophagolysosomes in dengue virus 3 production in HepG2 cells.自噬溶酶体在登革病毒3型于HepG2细胞中产生过程中的作用。
J Gen Virol. 2009 May;90(Pt 5):1093-1103. doi: 10.1099/vir.0.007914-0. Epub 2009 Mar 4.
10
Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis.登革热病毒非结构蛋白 3 将脂肪酸合酶重新分配到病毒复制部位，并增加细胞脂肪酸合成。
Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17345-50. doi: 10.1073/pnas.1010811107. Epub 2010 Sep 20.
引用本文的文献
1
Recapitulating dengue virus infection with human pluripotent stem cell-derived liver organoids for antiviral screening.利用人多能干细胞衍生的肝脏类器官重现登革病毒感染以进行抗病毒筛选。
Nat Commun. 2025 Aug 28;16(1):8069. doi: 10.1038/s41467-025-63323-3.
2
Kinesin light chain 1 interacts with NS1 and is a susceptibility factor for dengue virus infection in mosquito cells.驱动蛋白轻链1与NS1相互作用，是蚊细胞中登革病毒感染的一个易感因素。
J Gen Virol. 2025 Jul;106(7). doi: 10.1099/jgv.0.002132.
3
Mammalian fatty acid synthase: a commonly used viral host dependency factor and a putative target for host-targeted broad-spectrum antiviral therapeutic development.哺乳动物脂肪酸合酶：一种常用的病毒宿主依赖因子以及宿主靶向广谱抗病毒治疗研发的潜在靶点。
mBio. 2025 Aug 13;16(8):e0395424. doi: 10.1128/mbio.03954-24. Epub 2025 Jun 25.
4
Newcastle disease virus induces degradation of folliculin to regulate host cell energy metabolism and facilitate viral replication.新城疫病毒诱导卵泡抑素降解以调节宿主细胞能量代谢并促进病毒复制。
Poult Sci. 2025 May 23;104(8):105314. doi: 10.1016/j.psj.2025.105314.
5
Trehalose supports the growth of Aedes aegypti cells and modifies gene expression and dengue virus type 2 replication.海藻糖支持埃及伊蚊细胞的生长，并改变基因表达和2型登革病毒的复制。
PLoS Pathog. 2025 May 6;21(5):e1012795. doi: 10.1371/journal.ppat.1012795. eCollection 2025 May.
6
A portrait of the infected cell: how SARS-CoV-2 infection reshapes cellular processes and pathways.感染细胞的画像：严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染如何重塑细胞过程和信号通路。
Npj Viruses. 2024 Dec 17;2(1):66. doi: 10.1038/s44298-024-00076-8.
7
Investigating how dengue virus-induced metabolic changes affect the host immune response and how to develop Immunomodulatory strategies.研究登革病毒诱导的代谢变化如何影响宿主免疫反应以及如何制定免疫调节策略。
Virol J. 2025 Apr 25;22(1):117. doi: 10.1186/s12985-025-02745-3.
8
Identification of visible and near-infrared signature peaks for arboviruses and Plasmodium falciparum.鉴定虫媒病毒和恶性疟原虫的可见及近红外特征峰。
PLoS One. 2025 Apr 17;20(4):e0321362. doi: 10.1371/journal.pone.0321362. eCollection 2025.
9
Kinesin light chain 1 (KLC1) interacts with NS1 and is a susceptibility factor for dengue virus infection in mosquito cells.驱动蛋白轻链1（KLC1）与NS1相互作用，是蚊虫细胞中登革病毒感染的一个易感因素。
bioRxiv. 2025 Mar 20:2025.03.20.644413. doi: 10.1101/2025.03.20.644413.
10
The interaction of Orthoflavivirus nonstructural proteins 3 and 5 with human fatty acid synthase.正黄病毒非结构蛋白3和5与人脂肪酸合酶的相互作用。
PLoS One. 2025 Mar 25;20(3):e0319207. doi: 10.1371/journal.pone.0319207. eCollection 2025.
本文引用的文献
1
Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis.登革热病毒非结构蛋白 3 将脂肪酸合酶重新分配到病毒复制部位，并增加细胞脂肪酸合成。
Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17345-50. doi: 10.1073/pnas.1010811107. Epub 2010 Sep 20.
2
Enhanced hepatitis C virus genome replication and lipid accumulation mediated by inhibition of AMP-activated protein kinase.抑制 AMP 激活的蛋白激酶可增强丙型肝炎病毒基因组复制和脂质积累。
Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11549-54. doi: 10.1073/pnas.0912426107. Epub 2010 Jun 7.
3
Viral reorganization of the secretory pathway generates distinct organelles for RNA replication.病毒对分泌途径的重新组织为 RNA 复制生成了独特的细胞器。
Cell. 2010 May 28;141(5):799-811. doi: 10.1016/j.cell.2010.03.050.
4
Gene expression profiling indicates the roles of host oxidative stress, apoptosis, lipid metabolism, and intracellular transport genes in the replication of hepatitis C virus.基因表达谱分析表明，宿主氧化应激、细胞凋亡、脂质代谢和细胞内转运基因在丙型肝炎病毒复制中起作用。
J Virol. 2010 May;84(10):5404-14. doi: 10.1128/JVI.02529-09. Epub 2010 Mar 3.
5
Potential roles for cellular cofactors in hepatitis C virus replication complex formation.细胞辅助因子在丙型肝炎病毒复制复合体形成中的潜在作用。
Commun Integr Biol. 2009 Nov;2(6):471-3. doi: 10.4161/cib.2.6.9261.
6
Temporal proteome and lipidome profiles reveal hepatitis C virus-associated reprogramming of hepatocellular metabolism and bioenergetics.时间蛋白质组学和脂质组学图谱揭示丙型肝炎病毒相关的肝细胞代谢和生物能量重编程。
PLoS Pathog. 2010 Jan;6(1):e1000719. doi: 10.1371/journal.ppat.1000719. Epub 2010 Jan 8.
7
Dengue virus capsid protein usurps lipid droplets for viral particle formation.登革病毒衣壳蛋白利用脂滴进行病毒粒子的形成。
PLoS Pathog. 2009 Oct;5(10):e1000632. doi: 10.1371/journal.ppat.1000632. Epub 2009 Oct 23.
8
A genome-wide genetic screen for host factors required for hepatitis C virus propagation.一项针对丙型肝炎病毒传播所需宿主因子的全基因组遗传筛选。
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16410-5. doi: 10.1073/pnas.0907439106. Epub 2009 Aug 27.
9
The autophagy machinery is required to initiate hepatitis C virus replication.自噬机制是启动丙型肝炎病毒复制所必需的。
Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14046-51. doi: 10.1073/pnas.0907344106. Epub 2009 Aug 3.
10
Lipids in host-pathogen interactions: pathogens exploit the complexity of the host cell lipidome.在宿主-病原体相互作用中：病原体利用宿主细胞脂类组的复杂性。
Prog Lipid Res. 2010 Jan;49(1):1-26. doi: 10.1016/j.plipres.2009.07.003. Epub 2009 Jul 26.
Zotero 插件