Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.
Poult Sci. 2010 Dec;89(12):2634-9. doi: 10.3382/ps.2010-00938.
An experiment using 336 Hy-Line W-36 Single Comb White Leghorn hens was conducted to evaluate transgenic soybeans containing the gm-fad2-1 gene fragment and the gm-hra gene. Transcription of the gm-fad2-1 gene fragment results in an increased level of oleic acid (18:1) in the seed, and expression of the soybean acetolactate synthase protein (GM-HRA) encoded by the modified gm-hra gene, is used as a selectable marker during transformation. Pullets (20 wk of age) were placed in cage lots (7 hens/cage, 2 cages/lot) and were randomly assigned to 1 of 4 corn-soybean meal dietary treatments (6 lots/treatment) formulated with the following soybean meals: nontransgenic near-isoline control (control), nontransgenic commercial reference soybean meal A (92M72), nontransgenic commercial reference soybean meal B (93B15), or transgenic soybean meal produced from soybeans containing event DP-3Ø5423-1 (305423). Weeks 20 to 24 were a preconditioning period, and the 4 experimental diets were then fed from 25 to 36 wk of age. Differences between the 305423 and control group means were evaluated, with statistical significance at P < 0.05. Body weight, hen-day egg production, egg mass, feed consumption, and feed efficiency for hens fed the 305423 soybean meal were not significantly different from the respective values for hens fed diets formulated with the near-isoline soybean meal. Likewise, egg component weights, Haugh unit measures, and egg weights were similar regardless of the soybean meal source. This research indicates that performance of hens fed diets containing 305423 soybean meal, as measured by egg production and egg quality, was similar to that of hens fed diets formulated with the near-isoline control and commercial soybean meals.
一项使用 336 只海兰 W-36 单冠白来航母鸡的实验，评估了含有 gm-fad2-1 基因片段和 gm-hra 基因的转基因大豆。gm-fad2-1 基因片段的转录导致种子中油酸（18:1）水平升高，而修饰的 gm-hra 基因编码的大豆乙酰乳酸合酶蛋白（GM-HRA）的表达，在转化过程中作为选择性标记。育成母鸡（20 周龄）被放置在笼位（7 只/笼，2 笼/位）中，并随机分配到 4 种玉米-豆粕日粮处理（6 个处理/笼）中的 1 种，这些日粮由以下大豆粉配制而成：非转基因近等基因对照（对照）、非转基因商业参考大豆粉 A（92M72）、非转基因商业参考大豆粉 B（93B15）或含有事件 DP-3Ø5423-1 的转基因大豆粉（305423）。第 20 周到第 24 周为预适应期，然后从第 25 周到第 36 周饲喂 4 种实验日粮。评估了 305423 和对照组平均值之间的差异，P<0.05 时具有统计学意义。饲喂 305423 大豆粉母鸡的体重、母鸡日蛋产量、蛋重、饲料消耗和饲料效率与饲喂近等基因大豆粉日粮的母鸡的相应值无显著差异。同样，无论大豆粉来源如何，蛋成分重量、哈格单位测量值和蛋重都相似。本研究表明，通过产蛋量和蛋品质衡量，饲喂含有 305423 大豆粉日粮母鸡的性能与饲喂近等基因对照和商业大豆粉日粮母鸡的性能相似。
