Zhong R Q, Chen L, Gao L X, Zhang L L, Yao B, Yang X G, Zhang H F
1State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,No. 2 Yuanmingyuan West Road,Haidian District,Beijing 100193,China.
2Key Laboratory for Feed Biotechnology of the Ministry of Agriculture,Feed Research Institute,Chinese Academy of Agricultural Sciences,No. 12 Zhongguancun South Street,Haidian District,Beijing 100081,China.
Animal. 2016 Aug;10(8):1280-7. doi: 10.1017/S1751731116000203. Epub 2016 Feb 26.
The objective of the present study was to investigate the effect of feeding two transgenic corn lines containing the mCry1Ac gene from Bacillus thuringiensis strain (BT-799) and the maroACC gene from Agrobacterium tumefaciens strain (CC-2), respectively, on growth, egg quality and organ health indicators. Expression of the mCry1Ac gene confers resistance to Pyrausta nubilalis and the maroACC gene confers tolerance to herbicides. Healthy hens (n=96 placed in cages; 3 hens/cage) were randomly assigned to one of four corn-soybean meal dietary treatments (8 cages/treatment) formulated with the following corn: non-transgenic near-isoline control corn (control), BT-799 corn, CC-2 corn and commercially available non-transgenic reference corn (reference). The experiment was divided into three 4-week phases (week 1 to 4, week 5 to 8 and week 9 to 12), during which hens were fed mash diets. Performance (BW, feed intake and egg production) and egg quality were determined. Following slaughter at the end of 12 weeks of feeding (n=8/treatment), carcass yield and organ weights (heart, liver, spleen, lung, kidneys, stomach and ovary) were recorded; organs and intestines were sampled for histological analysis. Analysis of serum biochemistry parameters to assess the liver and kidney function were performed. No differences in BW, egg production and production efficiency were observed between hens consuming the control diet and hens consuming the BT-799 or CC-2 diet. Haugh unit measures and egg component weights were similar between the control and test groups. Carcass yield was not affected by the diet treatment. Similar organosomatic indices and serum parameters did not indicate the characteristics of organ dysfunction. All observed values of the BT-799 and CC-2 groups were within the calculated tolerance intervals. This research indicates that the performance, egg quality, organ health and carcass yield of laying hens fed diets containing the BT-799 or CC-2 corn line were similar to that of laying hens fed diets formulated with the non-transgenic near-isoline corn with comparable genetic backgrounds.
本研究的目的是调查分别饲喂含有来自苏云金芽孢杆菌菌株（BT - 799）的mCry1Ac基因和来自根癌农杆菌菌株（CC - 2）的maroACC基因的两种转基因玉米品系对生长、蛋品质和器官健康指标的影响。mCry1Ac基因的表达赋予对玉米螟的抗性，而maroACC基因赋予对除草剂的耐受性。将健康母鸡（n = 96，置于笼中；每笼3只母鸡）随机分配到四种玉米 - 豆粕日粮处理之一（每个处理8个笼子），日粮用以下玉米配制：非转基因近等基因系对照玉米（对照）、BT - 799玉米、CC - 2玉米和市售非转基因参考玉米（参考）。实验分为三个4周阶段（第1至4周、第5至8周和第9至12周），在此期间母鸡饲喂粉料日粮。测定生产性能（体重、采食量和产蛋量）和蛋品质。在饲喂12周结束时宰杀后（每个处理n = 8），记录胴体产量和器官重量（心脏、肝脏、脾脏、肺、肾脏、胃和卵巢）；采集器官和肠道样本进行组织学分析。进行血清生化参数分析以评估肝脏和肾脏功能。在采食对照日粮的母鸡与采食BT - 799或CC - 2日粮的母鸡之间，未观察到体重、产蛋量和生产效率的差异。哈夫单位测量值和蛋成分重量在对照组和试验组之间相似。日粮处理对胴体产量没有影响。相似的器官体指数和血清参数未表明器官功能障碍的特征。BT - 799和CC - 2组的所有观察值均在计算的耐受区间内。本研究表明，饲喂含有BT - 799或CC - 2玉米品系日粮的蛋鸡的生产性能、蛋品质、器官健康和胴体产量与饲喂具有可比遗传背景的非转基因近等基因系玉米日粮的蛋鸡相似。
