Ghaffariyeh Alireza, Honarpisheh Nazafarin, Heidari Mohammad Hossein, Puyan Sadollah, Abasov Fuad
Ophthalmology Department, Dr. Khodadoust Eye Hospital, Shiraz, Iran.
Optom Vis Sci. 2011 Jan;88(1):80-5. doi: 10.1097/OPX.0b013e3181fc329f.
To introduce a novel biomarker for screening of primary open-angle glaucoma (POAG) by detecting and measuring brain-derived neurotrophic factor (BDNF) in the serum of normal subjects and patients with early stage of glaucoma.
Twenty-five glaucoma patients as the case group and 25 age- and sex-matched normal persons as the control group were tested. The control group comprised 19 men and 6 women, with the mean age of 59.32 ± 11.8 years and without any apparent ocular or systemic diseases. The case group comprised 20 men and 5 women, with the mean age of 59.64 ± 11.56 years, who were assessed by routinely performed clinical and paraclinical investigations. BDNF levels in serum were determined by enzyme-linked immunosorbent assay using monoclonal antibodies specific for BDNF.
The mean of BDNF levels in the serum was 27.16 ± 5.53 ng/mL in the control subjects and 18.42 ± 4.05 ng/mL in the subjects with the early stage glaucoma. A statistically significant difference was evident between the two groups (p < 0.05). We found no significant differences in serum BDNF levels according to the subjects' age, gender, duration of the glaucoma, mean intraocular pressure, and blood pressure (p > 0.05). Glaucoma patients who had lower serum BDNF concentration had disclosed a significant negative correlation with pattern standard deviations.
We conclude that BDNF in the serum might be a useful biochemical marker for early detection of POAG. We also propose that this might be a reliable, time efficient, and cost-effective method for diagnosis, screening, and assessing the progression of POAG. However, more studies and trials are needed to investigate these factors in greater detail.
通过检测和测量正常受试者及青光眼早期患者血清中的脑源性神经营养因子（BDNF），引入一种用于筛查原发性开角型青光眼（POAG）的新型生物标志物。
对25例青光眼患者作为病例组和25例年龄及性别匹配的正常人作为对照组进行检测。对照组包括19名男性和6名女性，平均年龄为59.32±11.8岁，无任何明显的眼部或全身性疾病。病例组包括20名男性和5名女性，平均年龄为59.64±11.56岁，通过常规进行的临床和辅助临床检查进行评估。使用对BDNF具有特异性的单克隆抗体，通过酶联免疫吸附测定法测定血清中的BDNF水平。
对照组血清中BDNF水平的平均值为27.16±5.53 ng/mL，青光眼早期患者血清中BDNF水平的平均值为18.42±4.05 ng/mL。两组之间存在统计学上的显著差异（p<0.05）。我们发现血清BDNF水平在受试者的年龄、性别、青光眼病程、平均眼压和血压方面无显著差异（p>0.05）。血清BDNF浓度较低的青光眼患者与图形标准偏差呈显著负相关。
我们得出结论，血清中的BDNF可能是早期检测POAG的有用生化标志物。我们还提出，这可能是一种用于诊断、筛查和评估POAG进展的可靠、省时且经济高效的方法。然而，需要更多的研究和试验来更详细地研究这些因素。
