Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA.
Phytother Res. 2011 Jun;25(6):789-95. doi: 10.1002/ptr.3332. Epub 2010 Nov 12.
The purpose of this study was to characterize the putative anxiolytic-like activity of fractions prepared from a hydroethanol extract of Passiflora incarnata L. using the elevated plus-maze (EPM) in mice. The fractions were prepared as published recently, yielding a butanol, petroleum ether and chloroform fraction. From the tested fractions, the butanol fraction showed significant increases in the number of open arm entries in the EPM in concentrations of 2.1 mg/kg and 4.2 mg/kg corresponding to 150 and 300 mg/kg of the original extract. The highest activity was found for the chloroform fraction in doses of 0.17 mg/kg (10.0 ± 1.9, p < 0.001) and 0.34 mg/kg (6.6 ± 0.86; p < 0.05) which corresponds to a total extract dose of 150 and 300 mg/kg, respectively. Interestingly, the petroleum ether fraction did not show any effects in the elevated plus maze. A sedative or stimulatory effect of each of the fractions could be excluded, since none of the compounds had an influence on the total distance that the animals covered during the observation period. The results suggest that the active principle of passion flower seems to be in the chloroform fraction and to a lower extent in the butanol fraction.
本研究旨在利用小鼠高架十字迷宫（EPM）来描述西番莲（Passiflora incarnata L.）水醇提取物的各馏分所具有的潜在抗焦虑样活性。这些馏分是按照最近发表的方法制备的，得到了正丁醇、石油醚和氯仿馏分。在测试的馏分中，正丁醇馏分在 2.1mg/kg 和 4.2mg/kg 浓度下，分别相当于原始提取物 150mg/kg 和 300mg/kg 时，显著增加了 EPM 中进入开放臂的次数。氯仿馏分在 0.17mg/kg（10.0 ± 1.9，p < 0.001）和 0.34mg/kg（6.6 ± 0.86；p < 0.05）剂量下表现出最高的活性，分别相当于 150mg/kg 和 300mg/kg 的总提取物剂量。有趣的是，石油醚馏分在高架十字迷宫中没有显示出任何作用。由于这些化合物对动物在观察期间所覆盖的总距离没有影响，因此可以排除每个馏分具有镇静或刺激作用。结果表明，西番莲的活性成分似乎存在于氯仿馏分中，在正丁醇馏分中含量较低。
