Maślanka T, Jaroszewski J J, Markiewicz W, Jakubowski P
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Pol J Vet Sci. 2010;13(1):3-12.
The aim of the study was to determine whether treatment with recommended doses of meloxicam or flunixin had an effect on the apoptosis of peripheral blood T lymphocytes in calves. The study was carried out on 4-5 months old calves (n = 24, 8 per group). Experimental animals were injected subcutaneously with a single dose of 0.5 mg x kg(-1) of meloxicam or intravenously with 3 doses of 2.2 mg x kg(-1) day(-1) of flunixin. The non-treatment animals served as control. Blood samples were taken at day 0 and at days 1, 2, 3, 5, 7 and 14 after the first NSAIDs injection. Apoptosis was determined by flow cytometry using Annexin V-PE/7-AAD staining. The kinetic analysis of apoptosis in the total lymphocyte population, as well as in the CD4+ and CD8+ subsets did not reveal significant differences in the frequency of early apoptotic cells between control and experimental groups throughout the period studied. Although, 24 h after administration of the first dose of NSAIDs, late-stage apoptosis/necrosis was significantly increased in the total lymphocyte population (the meloxicam group), as well as in the CD4+ (the meloxicam group and the flunixin group) and CD8+ (the flunixin group) subsets of T cells. However, this disturbance was transient, relatively poorly expressed and, thus, unlikely to be of clinical significance. Our results indicate that the use of meloxicam or flunixin in accordance with the recommended dosage regimen in cattle do not have a clinically significant influence on apoptosis of peripheral blood T cells.
本研究的目的是确定用推荐剂量的美洛昔康或氟尼辛治疗是否对犊牛外周血T淋巴细胞的凋亡有影响。研究对象为4 - 5月龄的犊牛（n = 24，每组8头）。实验动物皮下注射单剂量0.5 mg·kg⁻¹的美洛昔康，或静脉注射3剂量2.2 mg·kg⁻¹·d⁻¹的氟尼辛。未治疗的动物作为对照。在首次注射非甾体抗炎药后的第0天以及第1、2、3、5、7和14天采集血样。使用膜联蛋白V-PE/7-AAD染色通过流式细胞术测定凋亡情况。在整个研究期间，对总淋巴细胞群体以及CD4⁺和CD8⁺亚群中凋亡的动力学分析未发现对照组和实验组早期凋亡细胞频率有显著差异。尽管在首次注射非甾体抗炎药24小时后，总淋巴细胞群体（美洛昔康组）以及T细胞的CD4⁺（美洛昔康组和氟尼辛组）和CD8⁺（氟尼辛组）亚群中的晚期凋亡/坏死显著增加。然而，这种干扰是短暂的，表达相对较弱，因此不太可能具有临床意义。我们的结果表明，按照推荐的给药方案在牛中使用美洛昔康或氟尼辛对外周血T细胞的凋亡没有临床显著影响。
