Maeda Yousuke, Tanaka Rie, Ohtsuka Hiromichi, Matsuda Keiici, Tanabe Taishi, Oikawa Masaaki
School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
J Vet Med Sci. 2011 Jul;73(7):957-60. doi: 10.1292/jvms.10-0422. Epub 2011 Mar 22.
This study compared the immunosuppressive effects of dexamethasone (DEX), flunixin meglumine (FLU) and meloxicam (MEL) on the peripheral blood mononuclear cells (PBMCs) of seven healthy Holstein calves in vitro. DEX significantly inhibited lymphocyte proliferation and expression of interferon (IFN)-γ, interleukin (IL)-2 and IL-4 messenger RNA (mRNA) in comparison with FLU and MEL. FLU and MEL dose-dependently inhibited lymphocyte proliferation, but did not significantly reduce mRNA expression. Our in vitro study indicates that steroidal anti-inflammatory drugs (SAIDs) as well as nonsteroidal anti-inflammatory drugs (NSAIDs) have immunosuppressive effects on calf PBMCs. These findings are important for assessing the indications and complications of NSAIDs in calves.
本研究在体外比较了地塞米松（DEX）、氟尼辛葡甲胺（FLU）和美洛昔康（MEL）对7头健康荷斯坦犊牛外周血单个核细胞（PBMCs）的免疫抑制作用。与FLU和MEL相比，DEX显著抑制淋巴细胞增殖以及干扰素（IFN）-γ、白细胞介素（IL）-2和IL-4信使核糖核酸（mRNA）的表达。FLU和MEL剂量依赖性地抑制淋巴细胞增殖，但未显著降低mRNA表达。我们的体外研究表明，甾体类抗炎药（SAIDs）以及非甾体类抗炎药（NSAIDs）对犊牛PBMCs均有免疫抑制作用。这些发现对于评估NSAIDs在犊牛中的适应证和并发症具有重要意义。
