Maślanka Tomasz, Jaroszewski Jerzy Jan
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn 10-718, Poland.
J Vet Sci. 2013;14(2):125-34. doi: 10.4142/jvs.2013.14.2.125. Epub 2013 Apr 30.
The purpose of this study was to evaluate the effect of meloxicam (MEL) on selected immune parameters of bovine CD25(high)CD4+, CD25(low)CD4+, and CD25-CD4+ cells. Peripheral blood mononuclear cells (PBMCs) collected from 12-month-old heifers were treated with MEL at a concentration corresponding to the serum level of this medication following administration at the recommended dose (MEL 5 × 10(⁻6) M) and at a concentration 10 times lower (MEL 5 × 10(⁻7) M). After 12 and 24 h of incubation with the drug, the percentage of CD25(high)CD4+ cells decreased; however, this disturbance was quickly reversed. Furthermore, the absolute number of CD25(high)CD4+ cells in the PBMC populations treated with MEL 5 × 10(⁻6) M for 48 and 168 h was increased. Prolonged (168 h) exposure to the drug increased the percentage of Foxp3+ cells in the CD25(high)CD4+ cell subpopulation. The higher dose of MEL was found to significantly increase the percentage of IFN-γ+ cells among the CD25-CD4+ cells. These results indicated that MEL does not exert an immunosuppressive effect by depleting CD4+ cells and suppression of IFN-γ+ production by these cells. Furthermore, IL-10 and TGF-β production was not changed following exposure to MEL.
本研究的目的是评估美洛昔康（MEL）对牛CD25(高)CD4+、CD25(低)CD4+和CD25-CD4+细胞的选定免疫参数的影响。从12月龄小母牛采集的外周血单个核细胞（PBMC），分别用相当于推荐剂量给药后该药物血清水平的浓度（MEL 5×10⁻⁶ M）和美洛昔康浓度低10倍（MEL 5×10⁻⁷ M）进行处理。在用药物孵育12小时和24小时后，CD25(高)CD4+细胞的百分比下降；然而，这种紊乱很快得到逆转。此外，用MEL 5×10⁻⁶ M处理48小时和168小时的PBMC群体中CD25(高)CD4+细胞的绝对数量增加。长时间（168小时）暴露于该药物可增加CD25(高)CD4+细胞亚群中Foxp3+细胞的百分比。发现较高剂量的MEL可显著增加CD25-CD4+细胞中IFN-γ+细胞的百分比。这些结果表明，MEL不会通过消耗CD4+细胞和抑制这些细胞产生IFN-γ+来发挥免疫抑制作用。此外，暴露于MEL后，IL-10和TGF-β的产生没有变化。
