Gebriel Mohammed, Uleberg Kai-Erik, Larssen Eivind, Bjørnstad Anne Hjelle, Sivertsvik Morten, Møller Simon Geir
Centre for Organelle Research, University of Stavanger, Stavanger, Norway.
J Agric Food Chem. 2010 Dec 8;58(23):12307-12. doi: 10.1021/jf103009r. Epub 2010 Nov 15.
Because Atlantic cod (Gadus morhua) has high economic value and its protein-rich muscle tissue is a food source, an increased understanding of the effects and consequences of environmental, nutritional, biological, and industrial factors on meat quality is necessary. To gain insight into cod muscle tissue protein composition, a large-scale proteomics approach has been used. One-dimensional polyacrylamide gel electrophoresis, nanoflow liquid chromatography peptide separation, and linear trap quadrupole mass spectrometry were used to identify 4804 peptides, which retrieved 9113 cod expressed sequence tags (ESTs), which in turn were mapped to 446 unique proteins. The same data set identified 3924 proteins from the zebrafish protein database, which highlights the complementary value of the two approaches. The generated data sets will act as a foundation for studies related to physiological status assessment of cod under different environmental conditions, screening for diseases, and biomarker identification for assessment of fish quality during industrial processing and preservation.
由于大西洋鳕鱼（Gadus morhua）具有较高的经济价值，其富含蛋白质的肌肉组织是一种食物来源，因此有必要进一步了解环境、营养、生物和工业因素对肉质的影响及后果。为深入了解鳕鱼肌肉组织的蛋白质组成，已采用大规模蛋白质组学方法。使用一维聚丙烯酰胺凝胶电泳、纳流液相色谱肽分离和线性阱四极杆质谱法鉴定了4804个肽段，这些肽段检索到9113个鳕鱼表达序列标签（EST），进而映射到446种独特蛋白质。同一数据集从斑马鱼蛋白质数据库中鉴定出3924种蛋白质，这突出了两种方法的互补价值。所生成的数据集将为不同环境条件下鳕鱼生理状态评估、疾病筛查以及工业加工和保存过程中鱼类质量评估的生物标志物鉴定等相关研究奠定基础。
