Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Mol Imaging Biol. 2011 Dec;13(6):1163-72. doi: 10.1007/s11307-010-0424-8.
This study aims to develop and characterize the trafficking of a dual-modal agent that identifies primary draining or sentinel lymph node (LN).
Herein, a dual-reporting silica-coated iron oxide nanoparticle (SCION) is developed. Nude mice were imaged by magnetic resonance (MR) and optical imaging and axillary LNs were harvested for histological analysis. Trafficking through lymphatics was observed with intravital and ex vivo confocal microscopy of popliteal LNs in B6-albino, CD11c-EYFP, and lys-EGFP transgenic mice.
In vivo, SCION allows visualization of LNs. The particle's size and surface functionality play a role in its passive migration from the intradermal injection site and its minimal uptake by CD11c+ dendritic cells and CD169+ and lys+ macrophages.
After injection, SCION passively migrates to LNs without macrophage uptake and then can be used to image LN(s) by MRI and fluorescence. Thus, SCION can potentially be developed for use in sentinel node resections or for intralymphatic drug delivery.
本研究旨在开发并表征一种可识别一级引流或前哨淋巴结（LN）的双模式示踪剂。
本研究中开发了一种双报告型硅基氧化铁纳米颗粒（SCION）。通过磁共振成像（MR）和光学成像对裸鼠进行成像，并对腋窝淋巴结进行组织学分析以获取淋巴结。通过活体和离体共聚焦显微镜观察 B6-albino、CD11c-EYFP 和 lys-EGFP 转基因小鼠的后肢淋巴结中的淋巴管内转运。
在体内，SCION 可用于可视化淋巴结。颗粒的大小和表面功能在其从皮内注射部位的被动迁移及其对 CD11c+树突状细胞和 CD169+和 lys+巨噬细胞的最小摄取中起作用。
注射后，SCION 可被动迁移至淋巴结而不被巨噬细胞摄取，然后可通过 MRI 和荧光成像来对淋巴结进行成像。因此，SCION 可能被开发用于前哨淋巴结切除或淋巴管内药物递送。
