Section de Chimie, Université de Lausanne, BCH, CH-1015, Lausanne, Switzerland.
J Biomol NMR. 1999 Jan;13(1):61-5. doi: 10.1023/A:1008307012647.
A novel one-dimensional NOE experiment is presented where a selected proton is excited by two-way heteronuclear cross- polarization between protons and nitrogen-15 or carbon-13. The utility of the method is demonstrated for a sample of 15N labeled human ubiquitin. Inter- and intra-residue NOEs are clearly observed in a very time-effective manner. The signal intensities can be easily normalized.
提出了一种新型的一维 NOE 实验，其中通过质子和氮-15 或碳-13 之间的双向异核交叉极化来激发选定的质子。该方法在 15N 标记的人泛素样品中得到了验证。以非常有效的方式清楚地观察到了残基间和残基内的 NOE。信号强度可以轻松归一化。
