Normalized one-dimensional NOE measurements in isotopically labeled macromolecules using two-way cross-polarization.

A novel one-dimensional NOE experiment is presented where a selected proton is excited by two-way heteronuclear cross- polarization between protons and nitrogen-15 or carbon-13. The utility of the method is demonstrated for a sample of 15N labeled human ubiquitin. Inter- and intra-residue NOEs are clearly observed in a very time-effective manner. The signal intensities can be easily normalized.