水-蛋白 NOEs：选择性水激发的优化方案。

Water-protein NOEs: Optimized scheme for selective water excitation.

机构信息

Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-171 77, Stockholm, Sweden.

出版信息

J Biomol NMR. 1999 Jan;13(1):73-6. doi: 10.1023/A:1008375214604.

Abstract

An alternative scheme for selective water excitation is proposed. The pulse sequence saturates the resonances from the solute, allowing the observation of water-solute NOEs with low artifact levels. The water resonance is subsequently excited by a relatively non-selective 90° pulse. The scheme is compared to other selective water excitation schemes. 2D NOE-NOESY and ROE-NOESY pulse sequences are proposed which afford high sensitivity by efficient water excitation and flip-back by radiation damping, yet allow the use of short mixing times for the buildup of water-solute NOEs.

摘要

提出了一种选择性水激发的替代方案。该脉冲序列使溶质的共振饱和，从而可以观察到低伪影水平的水-溶质 NOE。随后，通过相对非选择性的 90°脉冲激发水共振。该方案与其他选择性水激发方案进行了比较。提出了二维 NOE-NOESY 和 ROE-NOESY 脉冲序列，通过有效的水激发和辐射阻尼的翻转实现了高灵敏度，但允许使用短的混合时间来积累水-溶质 NOE。