Selective excitation of intense solvent signals in the presence of radiation damping.

Selective water excitation schemes are provided which rely on the radiation damping effect in probeheads characterized by high quality factors. The schemes are implemented in homonuclear NOE and ROE experiments, designed for the selective observation of water-protein cross peaks and their assignment using standard probeheads. The one-dimensional NOE and ROE experiments selectively record the cross section through the water signal usually measured in two-dimensional NOESY and ROESY spectra, and the two-dimensional NOE-NOESY and ROE-NOESY experiments selectively measure the cross section through the water line from 3D NOESY-NOESY and ROESY-NOESY spectra, respectively.