Melacini G, Kaptein R, Boelens R
Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, Utrecht, 3584 CH, The Netherlands.
J Magn Reson. 1999 Feb;136(2):214-8. doi: 10.1006/jmre.1998.1646.
An experimental approach for the editing of exchange-relayed NOEs in water-selective NOE experiments is presented. The proposed pulse sequence is based on the application during the NOE mixing time of continuous wave irradiation, which saturates resonances of relaying labile protons in slow chemical exchange with water. The technique can efficiently reduce the contributions of exchange-relayed NOE peaks that often crowd the water-selective NOE spectra and hide direct intermolecular NOEs between water and protein protons. The present approach opens new opportunities for the characterization of hydration by NMR, even in the proximity of polar labile groups.
本文提出了一种在水选择性核Overhauser效应（NOE）实验中编辑交换中继NOE的实验方法。所提出的脉冲序列基于在NOE混合时间应用连续波照射，该照射使与水进行缓慢化学交换的中继不稳定质子的共振饱和。该技术可以有效减少交换中继NOE峰的贡献，这些峰常常使水选择性NOE谱拥挤，并掩盖水与蛋白质质子之间的直接分子间NOE。即使在极性不稳定基团附近，本方法也为通过核磁共振（NMR）表征水合作用开辟了新的机会。
