School of Dental Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
J Periodontol. 2011 May;82(5):790-7. doi: 10.1902/jop.2010.100533. Epub 2010 Nov 16.
Cell-based therapy using autologous cells has been suggested as a potential approach for periodontal tissue regeneration. Spheroid systems are a form of three-dimensional cell culture that promotes cell matrix interaction, which could recapitulate the aspect of cell homeostasis in vivo. The aim of this study is to assess the interaction of periodontal fibroblast spheroids with synthetic and collagen-based membranes that have been used in guided tissue regeneration.
Commercially available normal human periodontal ligament fibroblasts were grown in spheroid forms using a liquid overlay technique and then transplanted onto a collagen-based and a polyglycolic acid-based membrane. The biologic interaction of the spheroids with the membranes was assessed using basic histology, Alamar blue tissue viability assay, scanning electron microscopy, and immunohistochemical analysis.
Periodontal fibroblast spheroids adhered to both membranes, and the cells were able to proliferate and migrate from the spheroids both horizontally and vertically into the membrane scaffolds. Immunohistochemical analysis showed expression of collagen type I, periostin, and Runx2 by the periodontal fibroblasts.
Periodontal fibroblast spheroids were able to grow three-dimensionally on the biologic membranes and may have the potential to be used together with guided tissue regeneration approaches as an adjunct for periodontal regeneration.
使用自体细胞的基于细胞的疗法已被提议作为牙周组织再生的一种潜在方法。球体系统是三维细胞培养的一种形式，可促进细胞基质相互作用，从而再现体内细胞动态平衡的方面。本研究旨在评估牙周成纤维细胞球体与已用于引导组织再生的合成和胶原基膜的相互作用。
使用液体覆盖技术将市售的正常人类牙周韧带成纤维细胞培养成球体形式，然后将其移植到胶原基和聚乙二醇酸基膜上。使用基础组织学、Alamar 蓝色组织活力测定、扫描电子显微镜和免疫组织化学分析评估球体与膜的生物学相互作用。
牙周成纤维细胞球体附着在两种膜上，细胞能够从球体水平和垂直地增殖和迁移到膜支架中。免疫组织化学分析显示牙周成纤维细胞表达Ⅰ型胶原、骨衬蛋白和 Runx2。
牙周成纤维细胞球体能够在生物膜上进行三维生长，并且可能有潜力与引导组织再生方法一起作为牙周再生的辅助手段。
