有丝分裂膜有助于集中和稳定有丝分裂纺锤体。
Mitotic membrane helps to focus and stabilize the mitotic spindle.
机构信息
Department of Electrical and Computer Engineering, The Johns Hopkins University, Baltimore, MD, USA.
出版信息
Biophys J. 2010 Nov 17;99(10):3182-90. doi: 10.1016/j.bpj.2010.09.053.
Abstract
During mitosis, microtubules (MTs), aided by motors and associated proteins, assemble into a mitotic spindle. Recent evidence supports the notion that a membranous spindle matrix aids spindle formation; however, the mechanisms by which the matrix may contribute to spindle assembly are unknown. To search for a mechanism by which the presence of a mitotic membrane might help spindle morphology, we built a computational model that explores the interactions between these components. We show that an elastic membrane around the mitotic apparatus helps to focus MT minus ends and provides a resistive force that acts antagonistically to plus-end-directed MT motors such as Eg5.
摘要
在有丝分裂过程中，微管（MTs）在马达和相关蛋白的辅助下组装成有丝分裂纺锤体。最近的证据支持这样一种观点，即一个膜性纺锤体基质有助于纺锤体的形成；然而，基质可能有助于纺锤体组装的机制尚不清楚。为了寻找有丝分裂膜存在的机制可能有助于纺锤体形态的形成，我们构建了一个计算模型来探索这些成分之间的相互作用。我们表明，有丝分裂装置周围的弹性膜有助于聚焦 MT 负端，并提供一种与 Eg5 等指向正端的 MT 马达相反的阻力。
相似文献
1
Mitotic membrane helps to focus and stabilize the mitotic spindle.
Biophys J. 2010 Nov 17;99(10):3182-90. doi: 10.1016/j.bpj.2010.09.053.
2
Reverse engineering of force integration during mitosis in the Drosophila embryo.
Mol Syst Biol. 2008;4:195. doi: 10.1038/msb.2008.23. Epub 2008 May 6.
3
Slide-and-cluster models for spindle assembly.
Curr Biol. 2007 Aug 21;17(16):1373-83. doi: 10.1016/j.cub.2007.07.058.
4
Computer simulations reveal motor properties generating stable antiparallel microtubule interactions.
J Cell Biol. 2002 Sep 16;158(6):1005-15. doi: 10.1083/jcb.200202051.
5
Mitosis, microtubules, and the matrix.
J Cell Biol. 2001 Jul 23;154(2):261-6. doi: 10.1083/jcb.200101097.
6
Microtubule patterning in the presence of moving motor proteins.
J Theor Biol. 2015 Oct 7;382:81-90. doi: 10.1016/j.jtbi.2015.06.040. Epub 2015 Jul 6.
7
Spatial regulation improves antiparallel microtubule overlap during mitotic spindle assembly.
Biophys J. 2008 Apr 1;94(7):2598-609. doi: 10.1529/biophysj.107.117671. Epub 2007 Dec 20.
8
Mechanisms of mitotic spindle assembly and function.
Int Rev Cytol. 2008;265:111-58. doi: 10.1016/S0074-7696(07)65003-7.
9
Mechanisms of chromosome biorientation and bipolar spindle assembly analyzed by computational modeling.
Elife. 2020 Feb 13;9:e48787. doi: 10.7554/eLife.48787.
10
The role of Patronin in Drosophila mitosis.
BMC Mol Cell Biol. 2019 Apr 17;20(Suppl 1):7. doi: 10.1186/s12860-019-0189-0.
引用本文的文献
1
Evidence for a role of spindle matrix formation in cell cycle progression by antibody perturbation.
PLoS One. 2018 Nov 28;13(11):e0208022. doi: 10.1371/journal.pone.0208022. eCollection 2018.
2
Membrane-based mechanisms of mitotic spindle assembly.
Commun Integr Biol. 2015 Nov 10;8(6):e1112473. doi: 10.1080/19420889.2015.1112473. eCollection 2015 Nov-Dec.
3
Commentary: Rab GTPase: A New Mitotic Delivery Service.
Front Cell Dev Biol. 2015 Nov 17;3:72. doi: 10.3389/fcell.2015.00072. eCollection 2015.
4
Modeling of Noisy Spindle Dynamics Reveals Separable Contributions to Achieving Correct Orientation.
Biophys J. 2015 Oct 6;109(7):1398-409. doi: 10.1016/j.bpj.2015.08.014.
5
An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region.
J Cell Biol. 2015 Aug 31;210(5):695-704. doi: 10.1083/jcb.201506107. Epub 2015 Aug 24.
6
A computational model for the formation of lamin-B mitotic spindle envelope and matrix.
Interface Focus. 2014 Jun 6;4(3):20130063. doi: 10.1098/rsfs.2013.0063.
7
Localized accumulation of tubulin during semi-open mitosis in the Caenorhabditis elegans embryo.
Mol Biol Cell. 2012 May;23(9):1688-99. doi: 10.1091/mbc.E11-09-0815. Epub 2012 Mar 7.
8
Mitotic moonlighting functions for membrane trafficking proteins.
Traffic. 2011 Jul;12(7):791-8. doi: 10.1111/j.1600-0854.2011.01184.x. Epub 2011 Mar 24.
9
Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix?
Chromosome Res. 2011 Apr;19(3):345-65. doi: 10.1007/s10577-011-9187-6.
本文引用的文献
1
Towards a quantitative understanding of mitotic spindle assembly and mechanics.
J Cell Sci. 2010 Oct 15;123(Pt 20):3435-45. doi: 10.1242/jcs.062208.
2
A membranous spindle matrix orchestrates cell division.
Nat Rev Mol Cell Biol. 2010 Jul;11(7):529-35. doi: 10.1038/nrm2919. Epub 2010 Jun 3.
3
Mitotic force generators and chromosome segregation.
Cell Mol Life Sci. 2010 Jul;67(13):2231-50. doi: 10.1007/s00018-010-0326-6. Epub 2010 Mar 10.
4
Stochastic simulation and graphic visualization of mitotic processes.
Methods. 2010 Jun;51(2):251-6. doi: 10.1016/j.ymeth.2010.01.021. Epub 2010 Jan 22.
5
Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope.
J Cell Biol. 2010 Jan 11;188(1):49-68. doi: 10.1083/jcb.200908150.
6
Mitotic microtubule crosslinkers: insights from mechanistic studies.
Curr Biol. 2009 Dec 15;19(23):R1089-94. doi: 10.1016/j.cub.2009.10.047.
7
Force and length in the mitotic spindle.
Curr Biol. 2009 Sep 15;19(17):R749-61. doi: 10.1016/j.cub.2009.07.028.
8
Dynein antagonizes eg5 by crosslinking and sliding antiparallel microtubules.
Curr Biol. 2009 Nov 17;19(21):1833-8. doi: 10.1016/j.cub.2009.09.025.
9
Membrane-induced bundling of actin filaments.
Nat Phys. 2008 Aug 31;4:789-793. doi: 10.1038/nphys1071.
10
A requirement for epsin in mitotic membrane and spindle organization.
J Cell Biol. 2009 Aug 24;186(4):473-80. doi: 10.1083/jcb.200902071.
Zotero 插件