有丝分裂微管交联剂：来自机制研究的见解。

Mitotic microtubule crosslinkers: insights from mechanistic studies.

机构信息

Department of Physics and Astronomy and Laser Centre, VU University, De Boelelaan 1081, 1081 HV Amsterdam, the Netherlands.

出版信息

Curr Biol. 2009 Dec 15;19(23):R1089-94. doi: 10.1016/j.cub.2009.10.047.

DOI:10.1016/j.cub.2009.10.047

PMID:20064413

Abstract

Mitosis depends on the mitotic spindle, a subcellular protein machine that uses dynamic microtubules and mitotic motors to assemble itself and to coordinate chromosome movements. Spindle function depends critically on the interplay of microtubule polymer dynamics and the motor proteins and non-motor microtubule-associated proteins (MAPs) that crosslink adjacent microtubules. These microtubule crosslinkers can organize microtubules into bundles with specific polarity patterns and some of them can slide adjacent microtubules in relation to one another. Here, we discuss the functions and mechanisms of action of three such crosslinkers: the motors kinesin-5 and kinesin-14, and the non-motor MAPs of the Ase1p family.

摘要

有丝分裂依赖于有丝分裂纺锤体，这是一种亚细胞蛋白机器，利用动态微管和有丝分裂马达来组装自身，并协调染色体运动。纺锤体的功能取决于微管聚合动力学以及马达蛋白和非马达微管相关蛋白（MAPs）之间的相互作用，这些蛋白交联相邻的微管。这些微管交联剂可以将微管组织成具有特定极性模式的束，其中一些可以使相邻的微管彼此相对滑动。在这里，我们讨论了三种这样的交联剂的功能和作用机制：马达蛋白 kinesin-5 和 kinesin-14，以及 Ase1p 家族的非马达 MAP。