Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
Curr Biol. 2009 Sep 15;19(17):R749-61. doi: 10.1016/j.cub.2009.07.028.
The mitotic spindle assembles to a steady-state length at metaphase through the integrated action of molecular mechanisms that generate and respond to mechanical forces. While molecular mechanisms that produce force have been described, our understanding of how they integrate with each other, and with the assembly/disassembly mechanisms that regulate length, is poor. We review current understanding of the basic architecture and dynamics of the metaphase spindle, and some of the elementary force-producing mechanisms. We then discuss models for force integration and spindle length determination. We also emphasize key missing data that notably include absolute values of forces and how they vary as a function of position within the spindle.
有丝分裂纺锤体通过产生和响应机械力的分子机制的综合作用，在中期组装到稳定的长度。虽然已经描述了产生力的分子机制，但我们对它们如何相互整合，以及与调节长度的组装/拆卸机制的整合，了解甚少。我们回顾了中期纺锤体的基本结构和动力学以及一些基本的力产生机制的现有认识。然后，我们讨论了力整合和纺锤体长度确定的模型。我们还强调了关键的缺失数据，特别是包括力的绝对值以及它们如何随纺锤体内部位置变化的信息。
