Gagliardi Christine, Bunnell Bruce A
Center for Stem Cell Research and Regenerative Medicine, Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA, USA.
Methods Mol Biol. 2011;702:3-16. doi: 10.1007/978-1-61737-960-4_1.
Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Rhesus monkeys are physiologically and phylogenetically similar to humans, and they and their cells are valuable for biomedical research and evaluation of preclinical therapies. Here, we describe methods for the isolation, culture, and differentiation of rhesus adipose-derived stem cells (rASCs).
脂肪组织是一种丰富且易于获取的干细胞来源,具有多能特性,适用于组织工程和再生医学应用。恒河猴在生理和系统发育上与人类相似,它们及其细胞对于生物医学研究和临床前治疗评估具有重要价值。在此,我们描述了恒河猴脂肪来源干细胞(rASCs)的分离、培养和分化方法。