Department of Bioengineering, University of California, Riverside, CA 92521, USA.
Biomolecules. 2022 Dec 7;12(12):1828. doi: 10.3390/biom12121828.
Photoaging is an important extrinsic aging factor leading to altered skin morphology and reduced function. Prior work has revealed a connection between photoaging and loss of subcutaneous fat. Currently, primary models for studying this are in vivo (human samples or animal models) or in vitro models, including human skin equivalents (HSEs). In vivo models are limited by accessibility and cost, while HSEs typically do not include a subcutaneous adipose component. To address this, we developed an "adipose-vascular" HSE (AVHSE) culture method, which includes both hypodermal adipose and vascular cells. Furthermore, we tested AVHSE as a potential model for hypodermal adipose aging via exposure to 0.45 ± 0.15 mW/cm 385 nm light (UVA). One week of 2 h daily UVA exposure had limited impact on epidermal and vascular components of the AVHSE, but significantly reduced adiposity by approximately 50%. Overall, we have developed a novel method for generating HSE that include vascular and adipose components and demonstrated potential as an aging model using photoaging as an example.
光老化是导致皮肤形态改变和功能下降的重要外在老化因素。先前的研究揭示了光老化与皮下脂肪丧失之间的联系。目前，研究这一现象的主要模型有体内（人体样本或动物模型）或体外模型，包括人皮肤等效物（HSE）。体内模型受到可及性和成本的限制，而 HSE 通常不包括皮下脂肪成分。为了解决这个问题，我们开发了一种“脂肪-血管”HSE（AVHSE）培养方法，其中包括真皮脂肪和血管细胞。此外，我们通过将 AVHSE 暴露于 0.45±0.15 mW/cm385nm 光（UVA）来测试其作为真皮脂肪老化的潜在模型。每天 2 小时，持续 1 周的 UVA 照射对 AVHSE 的表皮和血管成分影响有限，但显著减少了约 50%的脂肪量。总的来说，我们开发了一种生成包含血管和脂肪成分的 HSE 的新方法，并通过光老化作为示例，证明了其作为老化模型的潜力。
