Global DMPK, AstraZeneca R&D Södertälje, SE-151 85, Södertälje, Sweden.
Anal Chim Acta. 2011 Sep 9;701(2):119-28. doi: 10.1016/j.aca.2011.05.037. Epub 2011 Jun 23.
This tutorial provides an overview on a new technique for sample preparation, microextraction by packed sorbent (MEPS). Not only the automation process by MEPS is the advantage but also the much smaller volumes of the samples, solvents and dead volumes in the system. Other significant advantages such as the speed and the simplicity of the sample preparation process are provided. In this tutorial the main concepts of MEPS will be elucidated. Different practical aspects in MEPS are addressed. The factors affecting MEPS performance will be discussed. The application of MEPS in clinical and pre-clinical studies for quantification of drugs and metabolites in blood, plasma and urine will be provided. A comparison between MEPS and other extraction techniques such as SPE, LLE, SPME and SBSE will be discussed.
本教程提供了一种新的样品制备技术——填充固相微萃取（MEPS）的概述。不仅 MEPS 的自动化过程具有优势，而且样品、溶剂和系统死体积的体积也小得多。还提供了其他显著的优势，如样品制备过程的速度和简单性。在本教程中，将阐明 MEPS 的主要概念。讨论 MEPS 的不同实际方面。将讨论影响 MEPS 性能的因素。将提供 MEPS 在临床前和临床研究中用于定量血液、血浆和尿液中药物和代谢物的应用。将讨论 MEPS 与 SPE、LLE、SPME 和 SBSE 等其他萃取技术之间的比较。
