The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
PLoS One. 2010 Nov 15;5(11):e13986. doi: 10.1371/journal.pone.0013986.
Autologous transplanted fat has a high resorption rate, providing a clinical challenge for the means to reduce it. Erythropoietin (EPO) has non-hematopoietic targets, and we hypothesized that EPO may improve long-term fat graft survival because it has both pro-angiogenic and anti-apoptotic properties. We aimed to determine the effect of EPO on the survival of human fat tissue after its transplantation in nude mice.
METHODOLOGY/PRINCIPAL FINDINGS: Human fat tissue was injected subcutaneously into immunologically-compromised nude mice, and the grafts were then treated with either 20 IU or 100 IU EPO. At the end of the 15-week study period, the extent of angiogenesis, apoptosis, and histology were assessed in the fat grafts. The results were compared to vascular endothelial growth factor (VEGF)-treated and phosphate-buffered saline (PBS)-treated fat grafts. The weight and volume of the EPO-treated grafts were higher than those of the PBS-treated grafts, whose weights and volumes were not different from those of the VEGF-treated grafts. EPO treatment also increased the expression of angiogenic factors and microvascular density, and reduced inflammation and apoptosis in a dose-dependent manner in the fat grafts.
CONCLUSIONS/SIGNIFICANCE: Our data suggest that stimulation of angiogenesis by a cluster of angiogenic factors and decreased fat cell apoptosis account for potential mechanisms that underlie the improved long-term survival of fat transplants following EPO treatment.
自体移植脂肪吸收率高，这给降低吸收率的方法带来了临床挑战。促红细胞生成素（EPO）具有非造血靶标，我们假设 EPO 可能通过其具有促血管生成和抗细胞凋亡的特性来提高脂肪移植物的长期存活率。我们旨在确定 EPO 对裸鼠皮下注射人脂肪组织后其存活的影响。
方法/主要发现：将人脂肪组织注射到免疫缺陷的裸鼠皮下，然后用 20IU 或 100IU 的 EPO 处理移植物。在 15 周的研究结束时，评估脂肪移植物中的血管生成、细胞凋亡和组织学情况。将结果与血管内皮生长因子（VEGF）处理和磷酸盐缓冲盐水（PBS）处理的脂肪移植物进行比较。EPO 处理的移植物的重量和体积高于 PBS 处理的移植物，而 PBS 处理的移植物的重量和体积与 VEGF 处理的移植物无差异。EPO 处理还以剂量依赖的方式增加了脂肪移植物中血管生成因子的表达和微血管密度，并减少了炎症和细胞凋亡。
结论/意义：我们的数据表明，EPO 通过刺激一组血管生成因子和减少脂肪细胞凋亡来提高脂肪移植物的长期存活率，这可能是潜在的机制。
