Department of Aesthetic Plastic Surgery, Shanghai Eighth People's Hospital, Shanghai 200235, China.
Department of Anesthesia, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.
Biomed Res Int. 2021 Feb 16;2021:6613783. doi: 10.1155/2021/6613783. eCollection 2021.
Autologous fat grafting has been widely used for soft tissue filling in plastic surgery. Platelet-rich plasma (PRP) could play a wide role in health and disease because of containing a variety of growth factors and cytokines. Although previous studies have described the positive effect of autologous PRP mixed with fat grafts, only minimal improvements in fat graft survival have been reported. The present study is aimed at comparing the effects of PRP and platelet-poor plasma (PPP) on the survival and quality of fat grafting. We built a 180-day nude mouse model implanted with a fat graft supplemented with PRP, PPP, or saline, respectively. The above reagents (PRP, PPP, or saline) were injected two additional times after the initial engraftment. The survival ratio of the fat grafts and the capillary density in the PRP group were significantly higher than those in the PPP group and the saline group (control group) at 15, 30, 90, and 180 days posttransplantation ( < 0.05). The survival ratio of the PPP group was higher than that of the saline group ( < 0.05), but the capillary density in the PPP group was not significantly different from that in the saline group at any time point ( > 0.05). We hence conclude that the repeated application of PRP or PPP three times can enhance the survival of fat grafts within 180 days. Moreover, the effect of PRP is superior to that of PPP.
自体脂肪移植在整形手术中被广泛用于软组织填充。富血小板血浆 (PRP) 由于含有多种生长因子和细胞因子，因此在健康和疾病中具有广泛的作用。尽管先前的研究描述了自体 PRP 与脂肪移植物混合的积极作用，但仅报道了脂肪移植物存活率的微小改善。本研究旨在比较 PRP 和血小板贫浆 (PPP) 对脂肪移植存活率和质量的影响。我们建立了一个为期 180 天的裸鼠模型，分别植入补充有 PRP、PPP 或生理盐水的脂肪移植物。在初次移植后，另外两次注射上述试剂（PRP、PPP 或生理盐水）。在移植后 15、30、90 和 180 天，PRP 组的脂肪移植物存活率和毛细血管密度明显高于 PPP 组和生理盐水组（对照组）（<0.05）。PPP 组的脂肪移植物存活率高于生理盐水组（<0.05），但在任何时间点，PPP 组的毛细血管密度与生理盐水组均无显著差异（>0.05）。因此，我们得出结论，重复应用 PRP 或 PPP 三次可在 180 天内提高脂肪移植物的存活率。此外，PRP 的效果优于 PPP。
