大肠杆菌K-12半胱氨酸合成酶E基因座中第二个基因cysX的结构与表达

Structure and expression of cysX, the second gene in the Escherichia coli K-12 cysE locus.

作者信息

Tei H, Murata K, Kimura A

机构信息

Research Institute for Food Science, Kyoto University, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Mar 30;167(3):948-55. doi: 10.1016/0006-291x(90)90615-t.

DOI:10.1016/0006-291x(90)90615-t

PMID:2108679

Abstract

Protein products of the cysE region at 81 min on the chromosome of Escherichia coli K-12 (1) were analyzed by the maxicell method. Two kinds of polypeptides of molecular weight 33 K and 16 K were the products. The open reading frame (ORF) of the 33 K polypeptide consisted of 273 amino acids (Mr = 29,261). On the other hand, the 16 K ORF was overlapped by the opposite 33 K ORF and specified an extremely basic protein of 130 amino acids (Mr = 15,233). The gene coding for the 16 K polypeptide was named cysX. The expression of cysE and cysX in vivo was confirmed further by constructions of the cysE'-'lacZ and cysX'-'lacZ hybrid genes.

摘要

采用最大细胞法对大肠杆菌K-12染色体上81分钟处cysE区域的蛋白质产物进行了分析。得到了分子量分别为33K和16K的两种多肽产物。33K多肽的开放阅读框（ORF）由273个氨基酸组成（Mr = 29,261）。另一方面，16K的ORF与反向的33K ORF重叠，并编码一种由130个氨基酸组成的极端碱性蛋白质（Mr = 15,233）。编码16K多肽的基因被命名为cysX。通过构建cysE'-'lacZ和cysX'-'lacZ杂交基因，进一步证实了cysE和cysX在体内的表达。