Department of Environmental Immuno-Dermatology and Department of Molecular Pathology, Yokohama City University Graduate School of Medicine, Kanazawa-ku, Japan.
Br J Dermatol. 2011 Mar;164(3):560-7. doi: 10.1111/j.1365-2133.2010.10145.x. Epub 2011 Feb 17.
An increase in interleukin (IL)-18 production from epidermal cells has been reported in an atopic dermatitis (AD) mouse model, and subsequent topical application of Staphylococcus aureus results in severe dermatitis.
To reveal the relationship between S. aureus colonization of skin lesions and keratinocyte IL-18 production, particularly in AD with relatively low serum IgE levels. We also aimed to establish a simple and noninvasive method of assaying IL-18 produced by epidermal keratinocytes to evaluate local skin inflammation and therapeutic effects in patients with AD.
IL-18 in the horny layer of the skin was collected via a tape-stripping method and measured in 95 patients with AD and 40 healthy controls by enzyme-linked immunosorbent assay (ELISA). Clinical severity, blood data and S. aureus skin colonization were evaluated before and after treatment.
IL-18 levels in the horny layer were significantly higher in the skin lesions of patients with AD than in healthy controls and correlated with SCORAD, levels of serum IL-18, IgE, lactate dehydrogenase, thymus and activation-regulated chemokine, blood eosinophils and transepidermal water loss. In the AD group with serum IgE < 1500 IU mL(-1) , significantly higher IL-18 levels were observed in the horny layer of patients colonized with S. aureus compared with those who were not.
Epidermal IL-18 production was associated with the severity of AD. Staphylococcus aureus colonization seems to contribute to this IL-18 production, especially in the AD group with relatively low IgE production. Tape stripping provides an easy and noninvasive method to assess epidermal IL-18 production by ELISA.
在特应性皮炎（AD）小鼠模型中，表皮细胞的白细胞介素（IL）-18 产生增加，随后金黄色葡萄球菌（S. aureus）的局部应用导致严重的皮炎。
揭示皮肤病变部位金黄色葡萄球菌定植与角质形成细胞 IL-18 产生之间的关系，特别是在相对低血清 IgE 水平的 AD 中。我们还旨在建立一种简单且非侵入性的方法来检测表皮角质形成细胞产生的 IL-18，以评估 AD 患者的局部皮肤炎症和治疗效果。
通过胶带剥离法收集皮肤角质层中的 IL-18，并通过酶联免疫吸附试验（ELISA）在 95 例 AD 患者和 40 例健康对照者中进行测量。在治疗前后评估临床严重程度、血液数据和金黄色葡萄球菌皮肤定植情况。
AD 患者皮肤病变部位的角质层中 IL-18 水平明显高于健康对照组，且与 SCORAD、血清 IL-18、IgE、乳酸脱氢酶、胸腺和激活调节趋化因子、血液嗜酸性粒细胞和经皮水分丢失水平相关。在血清 IgE<1500 IU mL(-1)的 AD 组中，金黄色葡萄球菌定植的患者角质层中 IL-18 水平明显高于未定植的患者。
表皮 IL-18 产生与 AD 的严重程度相关。金黄色葡萄球菌定植似乎有助于这种 IL-18 产生，特别是在 IgE 产生相对较低的 AD 组中。胶带剥离提供了一种简单且非侵入性的方法，通过 ELISA 评估表皮 IL-18 产生。
