Fleischmann Leonardo, Crismani Adriano, Falkensammer Frank, Bantleon Hans-Peter, Rausch-Fan Xiaohui, Andrukhov Oleh
Division of Oral Biology, Bernhard Gottlieb School of Dentistry, Medical University of Vienna, Vienna, Austria.
J Mater Sci Mater Med. 2015 Jan;26(1):5335. doi: 10.1007/s10856-014-5335-9. Epub 2015 Jan 11.
In the present study we coated Ti surfaces with polytetrafluorethylene (PTFE) and titanium nitride (TiN) and investigated in vitro the behavior of osteoblasts on these surfaces. MG-63 osteoblasts were cultured on titanium discs with different surface treatment: uncoated Ti6Al4V, TiN-coated, PTFE-coated. Cell viability/proliferation was detected by MTT assay. Gene-expression levels of alkaline phosphatase (ALP), osteocalcin (OC), type I collagen, receptor activator of nuclear factor-kappa-B ligand (RANKL), and osteoprotegerin (OPG) were determined by qPCR. Cell behavior on different surfaces was observed by time-lapse microscopy. Cells grown on PTFE-coated Ti surface exhibited delayed surface attachment and decreased proliferation after 48 h. However, after 168 h of culture cells grown on PTFE-coated surface exhibited higher viability/proliferation, higher expression levels of ALP and OC, and higher OPG/RANKL ratio compared to uncoated surface. No effect of TiN-coating on any investigated parameter was found. Our results shows that PTFE coating exhibits no toxic effect on MG-63 cells and slightly stimulates expression of several genes associated with osteogenesis. We propose that PTFE coating could be considered as a possible choice for a surface treatment of temporary skeletal anchorage devices in orthodontics.
在本研究中，我们用聚四氟乙烯（PTFE）和氮化钛（TiN）涂覆钛表面，并在体外研究了成骨细胞在这些表面上的行为。将MG-63成骨细胞培养在经过不同表面处理的钛盘上：未涂覆的Ti6Al4V、TiN涂覆、PTFE涂覆。通过MTT法检测细胞活力/增殖。通过qPCR测定碱性磷酸酶（ALP）、骨钙素（OC）、I型胶原、核因子-κB受体激活剂配体（RANKL）和骨保护素（OPG）的基因表达水平。通过延时显微镜观察不同表面上的细胞行为。在PTFE涂覆的钛表面上生长的细胞在48小时后表现出延迟的表面附着和增殖减少。然而，在培养168小时后，与未涂覆表面相比，在PTFE涂覆表面上生长的细胞表现出更高的活力/增殖、更高的ALP和OC表达水平以及更高的OPG/RANKL比值。未发现TiN涂层对任何研究参数有影响。我们的结果表明，PTFE涂层对MG-63细胞没有毒性作用，并略微刺激了与成骨相关的几个基因的表达。我们建议，PTFE涂层可被视为正畸临时骨锚固装置表面处理的一种可能选择。
