The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China.
Mar Biotechnol (NY). 2011 Aug;13(4):706-12. doi: 10.1007/s10126-010-9332-9. Epub 2010 Nov 19.
A bacterial artificial chromosome (BAC) library of common carp Cyprinus carpio L. was constructed as a part of ongoing common carp genome project, which is aiming assembly of common carp genome. The library, containing a total of 92,160 BAC clones with an average insert size of 141 kb, was constructed into the restriction site of Hind III on BAC vector CopyControl pCC1BAC, covering 7.7 X haploid genome equivalents. Three dimension pools and superpools of the BAC library were established and 23 positive clones of 14 targets were identified from one-fifth of the BAC library. Pilot project of BAC end sequencing was conducted on 2,688 BAC ends from 1,344 clones and harvested 2,522 high-quality Q20 sequences with average length of 677 bp. The sequencing success rate was 93.8% and pair-end success rate was 92.3%. A total of 212 microsyntenies had been established between common carp and zebrafish genomes as a trial for genome-wide comparative genomics in these two closely related species.
鲤鱼（Cyprinus carpio L.）的细菌人工染色体（BAC）文库作为正在进行的鲤鱼基因组项目的一部分而构建，该项目旨在组装鲤鱼基因组。该文库共包含 92160 个 BAC 克隆，平均插入大小为 141kb，构建到 BAC 载体 CopyControl pCC1BAC 的 Hind III 限制位点上，覆盖了 7.7X 单倍体基因组当量。建立了 BAC 文库的三维池和超级池，并从五分之一的 BAC 文库中鉴定出 14 个目标的 23 个阳性克隆。对来自 1344 个克隆的 2688 个 BAC 末端进行了 BAC 末端测序试点项目，收获了 2522 个高质量的 Q20 序列，平均长度为 677bp。测序成功率为 93.8%，双端测序成功率为 92.3%。共建立了 212 个微同线性群，作为这两个密切相关物种全基因组比较基因组学的试验。
