Donti E, Lanfrancone L, Huebner K, Pascucci A, Venti G, Pengue G, Grignani F, Croce C M, Lania L, Pelicci P G
Istituto di Clinica Medica I, Policlinico Monteluce, Università di Perugia, Italy.
Hum Genet. 1990 Apr;84(5):391-5. doi: 10.1007/BF00195806.
The finger motif is a tandemly repeated DNA-binding domain recently identified in the primary structure of several eukaryotic transcriptional regulatory proteins. It has been proposed that some members of the finger-gene family are implicated in both normal cell proliferation and differentiation. We isolated several human finger genes by means of hybridization with a finger motif-containing DNA probe. One of these finger genes, HF.10, is expressed at low levels in a variety of human tissues and is down-regulated during the in vitro terminal differentiation of human leukemic myeloid cell lines. By in situ hybridization experiments and analysis of interspecific somatic cell hybrids we mapped the HF.10 gene to 3p21-22, a chromosome region frequently involved in karyotypic rearrangements associated with lung and renal cancer.
指状基序是最近在几种真核转录调节蛋白的一级结构中发现的串联重复DNA结合结构域。有人提出，指状基因家族的一些成员与正常细胞增殖和分化都有关。我们通过与含指状基序的DNA探针杂交分离出了几个人类指状基因。其中一个指状基因HF.10在多种人类组织中低水平表达，并且在人白血病髓系细胞系的体外终末分化过程中表达下调。通过原位杂交实验和种间体细胞杂种分析，我们将HF.10基因定位到3p21 - 22，这是一个经常参与与肺癌和肾癌相关的核型重排的染色体区域。
