Horibe K, Mashima Y, Tashiro T, Yamamori H, Okui K
First Department of Surgery, Chiba University School of Medicine, Japan.
JPEN J Parenter Enteral Nutr. 1990 Jan-Feb;14(1):56-9. doi: 10.1177/014860719001400156.
The capabilities of inline filters to retain bacteria and endotoxin were examined during simulated extended infusions for up to 168 hr. The tested inline filters were the ELD96 (Pall Biomedical Corp) and the IVEX 2 (Millipore Corp). Approximately 1 x 10(8) total cells of Escherichia coli B. were challenged to the upstream site of the filter. The test solution of 5% dextrose in water, 0.9% saline, Paremental A (A basic solution for total parenteral nutrition (TPN), a TPN solution in use in our clinic were infused continuously up to 168 hr and flow rate was maintained at 83 ml/hr. The effluents were analyzed using the Limulus Amebocyte Lysate (LAL) test to detect endotoxin and also passage of the challenged bacteria was tested at 24-hr intervals over 168 hr. The results were as follows: (1) The viability control culture showed the presence of viable bacteria throughout the 168-hr period of the experiment. (2) During the experiments, all filters produced sterile effluents. (3) LAL assay indicated that only the effluents from the ELD96 contained no detectable endotoxin for 168 hr.
在长达168小时的模拟延长输注过程中，对在线过滤器截留细菌和内毒素的能力进行了检测。所测试的在线过滤器为ELD96（颇尔生物医学公司）和IVEX 2（密理博公司）。将约1×10⁸个大肠杆菌B的总细胞接种到过滤器的上游部位。将5%葡萄糖水溶液、0.9%生理盐水、肠外营养A（一种用于全胃肠外营养（TPN）的基础溶液，我们诊所正在使用的一种TPN溶液）的测试溶液连续输注长达168小时，流速维持在83毫升/小时。使用鲎试剂法分析流出液以检测内毒素，并在168小时内每隔24小时测试一次被接种细菌的通过情况。结果如下：（1）活力对照培养显示在整个168小时的实验期间存在活细菌。（2）在实验过程中，所有过滤器产生的流出液均无菌。（3）鲎试剂法检测表明，只有ELD96的流出液在168小时内未检测到内毒素。
