Rusmin S, DeLuca P P
Am J Hosp Pharm. 1975 Apr;32(4):378-80.
A study was conducted on the effects of two antibiotics (gentamicin and carbenicillin) and of a sudden change from an isotonic to a hypotonic solution on the release of endotoxin by three gram-negative bacteria(Esherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) growing on a 0.22-mum pore size membrane filter. During a 72-hour constant flow of sterile lactated Ringers's solution through the contaminated filters, no endotoxin was released into the filtrates as tested by the coagulation of Limulus amebocyte lysate. However, flushing the filters with carbenicillin or gentamicin killed the bacteria and caused the release of endotoxin into the filtrates. A sudden osmotic change (flushing the filter with water) did not kill the bacteria nor cause the release of endotoxin into the filtrate.
针对两种抗生素（庆大霉素和羧苄青霉素）以及从等渗溶液突然转变为低渗溶液对生长在孔径为0.22微米的膜滤器上的三种革兰氏阴性菌（大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌）释放内毒素的影响进行了一项研究。在通过受污染滤器的无菌乳酸林格氏液持续流动72小时的过程中，通过鲎试剂凝结试验检测，滤液中未释放出内毒素。然而，用羧苄青霉素或庆大霉素冲洗滤器会杀死细菌，并导致内毒素释放到滤液中。突然的渗透压变化（用水冲洗滤器）不会杀死细菌，也不会导致内毒素释放到滤液中。
