Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, P.R. China.
Clin Chem Lab Med. 2011 Jan;49(1):55-60. doi: 10.1515/CCLM.2011.024. Epub 2010 Nov 22.
Coronary artery disease (CAD) is a complex multifactorial and polygenic disorder where multiple environmental and genetic factors are involved simultaneously. The purpose of this study was to explore the relationship between the interaction of cyclooxygenase-2 (COX-2) gene polymorphism and smoking and CAD in a Uighur population.
Using a case-control study of Chinese Uighur CAD patients (n=430) and healthy controls (n=470), we investigated the roles of G-765C polymorphism in the COX-2 gene (PTGS2) by the use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.
The PTGS2 GG genotype was significantly more prevalent in CAD patients (84.6% vs. 78.3%; p=0.014). Multiple logistic regression analysis showed two independent risk factors: smoking (OR 1.89, 95% CI 1.01-5.24; p=0.008) and hypertension (OR 2.73, 95% CI 1.59-7.21; p=0.001). Moreover, there was a synergistic effect between smoking and the PTGS2 polymorphism and the occurrence of CAD (interaction p=0.009). The odds ratio (OR) estimated by the combined analysis of the PTGS2 GG genotype and smoking history (OR 4.09, 95% CI 2.7-9.3) was markedly higher than that estimated separately from the PTGS2 GG genotype (OR 1.28, 95% CI 0.8-1.9) or smoking (OR 2.51, 95% CI 1.5-5.7) alone. Plasma 6-keto-PGF1α, a stable metabolite of PGI(2), was lower in individuals with the PTGS2 GG genotype (p<0.05). Smoking could further lower plasma 6-keto-PGF1α concentrations in GG genotype carriers than non-smokers, especially in patients with CAD.
The PTGS2 polymorphism and smoking were synergistically and significantly associated in Chinese Uighur patients with CAD.
冠心病（CAD）是一种复杂的多因素和多基因疾病，其中涉及多种环境和遗传因素。本研究的目的是探讨环氧化酶-2（COX-2）基因多态性与吸烟在维吾尔族人群中与 CAD 的关系。
采用维吾尔族 CAD 患者（n=430）和健康对照（n=470）的病例对照研究，应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析 COX-2 基因（PTGS2）的 G-765C 多态性。
PTGS2 GG 基因型在 CAD 患者中明显更为常见（84.6%比 78.3%；p=0.014）。多因素 logistic 回归分析显示两个独立的危险因素：吸烟（OR 1.89，95%CI 1.01-5.24；p=0.008）和高血压（OR 2.73，95%CI 1.59-7.21；p=0.001）。此外，吸烟与 PTGS2 多态性与 CAD 的发生之间存在协同作用（交互作用 p=0.009）。通过对 PTGS2 GG 基因型和吸烟史联合分析估计的比值比（OR）（4.09，95%CI 2.7-9.3）明显高于单独从 PTGS2 GG 基因型（OR 1.28，95%CI 0.8-1.9）或吸烟（OR 2.51，95%CI 1.5-5.7）估计的值。PGI2 的稳定代谢产物 6-酮-PGF1α在 PTGS2 GG 基因型个体中较低（p<0.05）。与不吸烟者相比，吸烟可进一步降低 GG 基因型携带者的血浆 6-酮-PGF1α浓度，尤其是在 CAD 患者中。
PTGS2 多态性与吸烟在中国维吾尔族 CAD 患者中具有协同显著相关性。
