Coppack S W, Frayn K N, Humphreys S M, Whyte P L, Hockaday T D
Sheikh Rashid Diabetes Unit, Radcliffe Infirmary, Oxford, United Kingdom.
Metabolism. 1990 Apr;39(4):384-90. doi: 10.1016/0026-0495(90)90253-9.
Measurements of arteriovenous differences across subcutaneous abdominal tissue (mainly adipose) and deep forearm tissue (mainly muscle) were made on 25 occasions in normal subjects after an overnight fast. Adipose tissue was shown to be strongly lipolytic (releasing nonesterified fatty acids and glycerol), to clear circulating triacylglycerol, glucose, ketone bodies and acetate, and to produce lactate. Uptake of circulating carbohydrate and ketones was sufficient to account for only 51% of the adipose tissue oxygen consumption, implying that adipose tissue utilizes fuel(s) stored within it. The mean fractional re-esterification rate of fatty acids in adipose tissue was 13% to 19%. Arteriovenous differences were converted to fluxes of carbon atoms to compare the movements of different fuels. (Amino acids were not included in these calculations.) Adipose tissue after an overnight fast was a net exporter of carbon, whereas in resting muscle the uptake of carbon atoms from circulating carbohydrate and lipid fuels approximately balanced the CO2 production. Fatty acids were the main form in which carbon left adipose tissue, and the main source of carbon atoms entering the resting forearm.
在正常受试者禁食过夜后，对25例受试者的腹部皮下组织（主要为脂肪组织）和前臂深部组织（主要为肌肉组织）进行了动静脉差异测量。结果显示，脂肪组织具有强烈的脂解作用（释放非酯化脂肪酸和甘油），能够清除循环中的三酰甘油、葡萄糖、酮体和乙酸盐，并产生乳酸。循环中的碳水化合物和酮体的摄取量仅足以满足脂肪组织耗氧量的51%，这意味着脂肪组织利用其自身储存的燃料。脂肪组织中脂肪酸的平均再酯化率为13%至19%。将动静脉差异转化为碳原子通量，以比较不同燃料的转运情况。（这些计算中未包括氨基酸。）禁食过夜后的脂肪组织是碳的净输出者，而在静息肌肉中，循环中的碳水化合物和脂质燃料中碳原子的摄取量与二氧化碳产生量大致平衡。脂肪酸是碳离开脂肪组织的主要形式，也是进入静息前臂的碳原子的主要来源。
