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单次鼻腔内给予 mDEF201（腺病毒载体鼠干扰素-α）可预防致死性 SARS-CoV BALB/c 小鼠模型的死亡。

Single-dose intranasal administration with mDEF201 (adenovirus vectored mouse interferon-alpha) confers protection from mortality in a lethal SARS-CoV BALB/c mouse model.

机构信息

Institute for Antiviral Research, Department of Animal, Dairy and Veterinary Science, 5600 Old Main Hill, Logan, Utah State University, Logan, UT 84322, USA.

出版信息

Antiviral Res. 2011 Jan;89(1):75-82. doi: 10.1016/j.antiviral.2010.11.007. Epub 2010 Nov 18.

DOI:10.1016/j.antiviral.2010.11.007
PMID:21093489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3018546/
Abstract

Interferons (IFNs) are a first line of defense against viral infection. Herein we describe the use of an adenovirus vectored mouse IFN alpha gene (mDEF201) as a prophylactic and treatment countermeasure in a SARS-CoV-infected BALB/c mouse model. Complete survival protection was observed in mice given a single dose of mDEF201 administered intranasally 1, 3, 5, 7, or 14 days prior to lethal SARS-CoV challenge (p<0.001), and body weights of these treated mice were unaffected by the challenge. In addition, low doses of mDEF201 protected lungs in a dose dependent manner as measured by a reduction in gross pathology. Intranasal treatment with mDEF201 ranging from 10(6) to 10(8)PFU significantly protected mice against a lethal SARS-CoV infection in a dose dependent manner up to 12h post infection (p<0.001). The data suggest that mDEF201 is a new class of antiviral agent further development as treatment for SARS-CoV infections.

摘要

干扰素 (IFNs) 是抵抗病毒感染的第一道防线。在此，我们描述了一种腺病毒载体的小鼠 IFNα 基因 (mDEF201) 作为一种预防和治疗 SARS-CoV 感染的 BALB/c 小鼠模型的对策。在 SARS-CoV 攻击前 1、3、5、7 或 14 天给予单剂量鼻腔内给予 mDEF201 的小鼠观察到完全的存活保护 (p<0.001)，并且这些治疗小鼠的体重不受攻击的影响。此外，低剂量的 mDEF201 通过减少大体病理学测量，以剂量依赖的方式保护肺部。从 10(6)到 10(8)PFU 的鼻腔内给予 mDEF201 治疗在感染后 12 小时内以剂量依赖的方式显著保护小鼠免受致死性 SARS-CoV 感染 (p<0.001)。数据表明 mDEF201 是一种新的抗病毒药物，进一步开发为治疗 SARS-CoV 感染的药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/a7d0066f75e0/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/2944703bb205/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/3862effde379/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/e0dc2282df42/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/83c361007f99/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/07af1d0a5f2d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/a7d0066f75e0/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/2944703bb205/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/3862effde379/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/e0dc2282df42/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/83c361007f99/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/07af1d0a5f2d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec6a/7114248/a7d0066f75e0/gr6.jpg

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