Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario M5S1A8, Canada.
Microbes Infect. 2011 Feb;13(2):189-97. doi: 10.1016/j.micinf.2010.10.023. Epub 2010 Nov 18.
Staphylococcus aureus promotes the onset and severity of atopic dermatitis (AD), which is exacerbated by superantigen toxins SEB and SEC. The genetic identity of these isolates, and their relationship to common hospital- or community-associated methicillin resistant S. aureus (HA-MRSA and CA-MRSA) has not been defined. We conducted spa typing, partial multi-locus sequence typing (MLST), and toxin profiling (seb, sec, lukS-PV) of S. aureus from 119 pediatric and 40 adult AD patients. MLST clonal complexes CC45, CC5, CC15, CC1, CC8 and CC30 accounted for 79% of isolates, representing the same major groups reported for nosocomial S. aureus in hospital intensive care units. The highest disease severity was associated with CC1, which was significantly greater relative to CC15 (p = 0.017) or CC30 (p = 0.040), but with no significant difference relative to CC45, CC5 or CC8. Although there were two few lukS-PV, seb or sec isolates to infer a role in disease severity, CC45 was identified as a source of SEC producing strains, and lukS-PVL was associated with a small number of CC5 pediatric isolates. CC1 harbored the only CA-MRSA that was identified, and was a source of isolates that expressed both seb and sec, and closely resembled the USA400 strain of CA-MRSA.
金黄色葡萄球菌可促进特应性皮炎（AD）的发病和严重程度，而超抗原毒素 SEB 和 SEC 则可加重 AD。这些分离株的遗传同一性及其与常见的医院或社区相关耐甲氧西林金黄色葡萄球菌（HA-MRSA 和 CA-MRSA）的关系尚未确定。我们对 119 例儿科和 40 例成人 AD 患者的金黄色葡萄球菌进行了 spa 分型、部分多位点序列分型（MLST）和毒素分析（seb、sec、lukS-PV）。MLST 克隆复合体 CC45、CC5、CC15、CC1、CC8 和 CC30 占分离株的 79%，代表了医院重症监护病房中报告的医院获得性金黄色葡萄球菌的主要群组。最高的疾病严重程度与 CC1 相关，与 CC15（p=0.017）或 CC30（p=0.040）相比，CC1 显著更高，但与 CC45、CC5 或 CC8 相比无显著差异。尽管 lukS-PV、seb 或 sec 分离株太少，无法推断其在疾病严重程度中的作用，但 CC45 被鉴定为 SEC 产生菌株的来源，而 lukS-PVL 与少数 CC5 儿科分离株有关。CC1 携带了唯一鉴定出的 CA-MRSA，是表达 seb 和 sec 的分离株的来源，并且与 CA-MRSA 的 USA400 菌株非常相似。
