Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria.
Mol Immunol. 2011 Jan;48(4):600-9. doi: 10.1016/j.molimm.2010.10.019. Epub 2010 Nov 21.
Allergies to certain fruits such as banana, avocado, chestnut and kiwi are described in 30-70% of latex-allergic patients. This association is attributed to the cross-reactivity between the major latex allergen hevein and hevein-like domains (HLDs) from fruit class I chitinases. We aimed to assess the extent of cross-reactivity between hevein and HLDs using sera from latex-allergic patients with and without plant food allergy. Hevein and HLDs of latex, banana, and avocado chitinases were expressed in Escherichia coli as fusion proteins with the maltose-binding protein and purified by affinity chromatography. IgE binding to these proteins was studied in sera from 59 latex-allergic patients and 20 banana-allergic patients without latex allergy by ELISA and ELISA inhibition. Additionally, 16,408 allergic patients' sera were tested for IgE binding to hevein, latex chitinase, and wheat germ agglutinin using an allergen microarray. Hevein-specific IgE was detected in 34/59 (58%) latex-allergic patients' sera. HLDs of latex, banana, and avocado chitinases were recognized by 21 (36%), 20 (34%), and 9 (15%) sera, respectively. In contrast, only one of 20 banana-allergic patients without latex allergy was sensitized to chitinase HLDs. In most tested latex-allergic patients' sera, IgE binding to hevein was only partially reduced by preincubation with HLDs. Among hevein-sensitized, latex-allergic patients, the percentage of plant food allergy (15/34 = 44%) was equal to latex-allergic patients without hevein sensitization (11/25 = 44%). In the general allergic population, 230 of 16,408 sera (1.4%) reacted to hevein and/or a hevein-like allergen. Of these, 128 sera showed an isolated sensitization to hevein, whereas only 17 bound to latex chitinase or wheat germ agglutinin without hevein sensitization. In conclusion, the IgE response to HLDs is elicited by hevein as sensitizing allergen in most cases. Despite considerable cross-reactivity between these allergens, no correlation between latex-associated plant food allergy and sensitization to hevein or HLDs was found.
某些水果（如香蕉、鳄梨、栗子和猕猴桃）的过敏反应在 30-70%的乳胶过敏患者中描述。这种关联归因于主要乳胶过敏原巴西橡胶树蛋白与水果 I 类几丁质酶的巴西橡胶树蛋白样结构域（HLD）之间的交叉反应性。我们旨在使用乳胶过敏患者的血清评估巴西橡胶树蛋白和 HLD 之间的交叉反应程度，这些患者有无植物性食物过敏。巴西橡胶树蛋白和 HLD 与乳胶、香蕉和鳄梨几丁质酶在大肠杆菌中作为麦芽糖结合蛋白的融合蛋白表达，并通过亲和层析纯化。通过 ELISA 和 ELISA 抑制，在 59 例乳胶过敏患者和 20 例无乳胶过敏的香蕉过敏患者的血清中研究了这些蛋白与 IgE 的结合。使用过敏原微阵列测试了 16408 例过敏患者血清中的巴西橡胶树蛋白、乳胶几丁质酶和麦胚凝集素与 IgE 的结合。在 34/59 例（58%）乳胶过敏患者的血清中检测到巴西橡胶树蛋白特异性 IgE。乳胶、香蕉和鳄梨几丁质酶的 HLD 分别被 21 （36%）、20 （34%）和 9 （15%）血清识别。相比之下，在 20 例无乳胶过敏的香蕉过敏患者中，只有 1 例对几丁质酶 HLD 过敏。在大多数测试的乳胶过敏患者的血清中，与 HLD 预孵育仅部分减少了对巴西橡胶树蛋白的 IgE 结合。在巴西橡胶树蛋白致敏的乳胶过敏患者中，植物性食物过敏的百分比（15/34=44%）与无巴西橡胶树蛋白致敏的乳胶过敏患者（11/25=44%）相同。在一般过敏人群中，在 16408 例血清中有 230 例（1.4%）对巴西橡胶树蛋白和/或巴西橡胶树蛋白样变应原反应。其中，128 例血清仅对巴西橡胶树蛋白呈孤立敏感性，而 17 例血清在没有巴西橡胶树蛋白致敏的情况下仅与乳胶几丁质酶或麦胚凝集素结合。总之，在大多数情况下，HLD 的 IgE 反应是由作为致敏变应原的巴西橡胶树蛋白引起的。尽管这些过敏原之间存在相当大的交叉反应性，但未发现乳胶相关植物性食物过敏与巴西橡胶树蛋白或 HLD 致敏之间存在相关性。
