Dudley D J, Mitchell M D, Branch D W
Department of Obstetrics and Gynecology, University of Utah, Salt Lake City.
Am J Obstet Gynecol. 1990 Apr;162(4):953-8; discussion 958-9. doi: 10.1016/0002-9378(90)91296-o.
Antiphospholipid antibodies are associated with recurrent pregnancy loss and thrombosis. Studies suggest that antiphospholipid antibodies may inhibit the production of prostacyclin by vascular tissues. We incubated sera from women with moderate to high levels of antiphospholipid antibodies with primary human umbilical vein endothelial cell cultures. Intact confluent, subconfluent, hydrogen peroxide-damaged, and mechanically damaged endothelial cell monolayers were evaluated for the production of the prostacyclin metabolite 6-keto-prostaglandin F1 alpha in the presence of sera that were positive and negative for antiphospholipid antibodies. All sera were assayed for baseline concentrations of 6-keto-prostaglandin F1 alpha before incubation with endothelial monolayers. Additionally, the extent of binding of antiphospholipid antibodies to intact and damaged endothelium was studied by immunofluorescent techniques and enzyme-linked immunosorbent assay. Our results indicate that prostacyclin production is not impaired by sera containing antiphospholipid antibodies, regardless of the condition of the endothelium. Further, we were unable to demonstrate binding of immunoglobulin from sera containing antiphospholipid antibodies to intact or damaged endothelium. However, the baseline concentration of 6-keto-prostaglandin F1 alpha was nearly fourfold higher in sera containing antiphospholipid antibodies. The finding of elevated baseline serum concentrations of prostacyclin metabolite in patients with antiphospholipid antibodies suggests a condition of long-term stimulation or altered metabolism of prostacyclin.
抗磷脂抗体与复发性流产和血栓形成有关。研究表明，抗磷脂抗体可能会抑制血管组织中前列环素的产生。我们将抗磷脂抗体水平为中度至高度的女性血清与原代人脐静脉内皮细胞培养物一起孵育。在存在抗磷脂抗体呈阳性和阴性的血清的情况下，评估完整的汇合、亚汇合、过氧化氢损伤和机械损伤的内皮细胞单层中前列环素代谢物6-酮-前列腺素F1α的产生。在与内皮单层孵育之前，对所有血清进行6-酮-前列腺素F1α的基线浓度测定。此外，通过免疫荧光技术和酶联免疫吸附测定研究抗磷脂抗体与完整和受损内皮的结合程度。我们的结果表明，无论内皮的状况如何，含有抗磷脂抗体的血清均不会损害前列环素的产生。此外，我们无法证明含有抗磷脂抗体的血清中的免疫球蛋白与完整或受损内皮的结合。然而，含有抗磷脂抗体的血清中6-酮-前列腺素F1α的基线浓度几乎高出四倍。抗磷脂抗体患者血清中前列环素代谢物基线浓度升高的发现表明存在前列环素长期刺激或代谢改变的情况。
