Gosling P, Dickson G
Biochemistry Department, Selly Oak Hospital, Birmingham, UK.
Ann Clin Biochem. 1990 Mar;27 ( Pt 2):147-51. doi: 10.1177/000456329002700211.
The mean arterial blood PO2 values of 20 patients' samples taken into Sarstedt 2 mL Monovette syringes were found to be initially 0.43 kPa higher and after 15 min storage 0.93 kPa higher than the mean values using Corning 3 mL Blood Gas Kit syringes. The Sarstedt syringe allowed entry of small air bubbles and the plunger was noted to be stiff. We speculated that high injection pressures might increase measured PO2. Using each type of syringe, 20 ITU staff injected saline into a Corning 178 pH/blood analyser linked to a pressure transducer via a three-way connector. Mean injection pressures for Sarstedt and Corning syringes were 17.15 and 6.42 kPa, respectively (P less than 0.005). Following injection at pressures between 2.8 and 26.6 kPa, the mean PO2 of equilibrated saline containing an air bubble was 0.80 kPa higher than the mean value obtained at injection pressures of less than 2.8 kPa. However, with no air bubble the mean PO2 of equilibrated saline was still 0.45 kPa higher when injected at pressures between 2.8 and 26.6 kPa than the mean value obtained with injection pressures of 2.8 kPa. We suggest that for optimal PO2 determinations syringes should not only allow minimal air contamination but also have plungers that reduce injection pressure to a minimum.
研究发现，将20位患者的样本采集到Sarstedt 2 mL单通道采血管中时，其平均动脉血氧分压（PO2）值最初比使用康宁3 mL血气试剂盒采血管时的平均值高0.43 kPa，储存15分钟后则高0.93 kPa。Sarstedt采血管允许小气泡进入，且发现其活塞较硬。我们推测高注射压力可能会增加所测的PO2值。20名重症监护室工作人员使用每种类型的采血管，通过三通连接器将盐水注入与压力传感器相连的康宁178型pH/血液分析仪中。Sarstedt采血管和康宁采血管的平均注射压力分别为17.15 kPa和6.42 kPa（P<0.005）。在2.8至26.6 kPa的压力下进行注射后，含有气泡的平衡盐水的平均PO2比在低于2.8 kPa的注射压力下获得的平均值高0.80 kPa。然而，对于无气泡的情况，在2.8至26.6 kPa的压力下注射时，平衡盐水的平均PO2仍比在2.8 kPa注射压力下获得的平均值高0.45 kPa。我们建议，为了获得最佳的PO2测定结果，采血管不仅应允许最小程度的空气污染，而且其活塞应将注射压力降至最低。
