D'Alessandro Brian, Dhawan Atam P
Department of Electrical and Computer Engineering, New Jersey Institute of Technology, Newark, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2010;2010:6637-40. doi: 10.1109/IEMBS.2010.5627145.
The early detection of melanoma is critical for patient survival. One of the indentifying features of new malignancy is increased blood flow to the lesion. Multispectral transillumination using the Nevoscope has been demonstrated to be an effective tool for imaging the sub-surface vascular architecture of skin lesions. Using multispectral images obtained from this tool in the visible and near-infrared range, as well as the relative difference in spectral absorption due to oxyhemoglobin and deoxyhemoglobin, we propose an empirical method to estimate the blood flow volume within a skin lesion. From the images, estimates of the distribution of both Hb and HbO(2) are calculated along with a ratiometric feature describing the relative oxygen saturation level in the blood. We validate our proposed method through the imaging of a skin phantom with embedded capillaries which can be filled with either an artificial Hb or HbO(2) liquid. Our near-IR, multispectral computations nicely differentiate the Hb filled phantom versus the HbO(2) filled phantom, demonstrating that these chromophores can be successfully separated and individually characterized for use in estimating the relative oxygen saturation of skin tissue.
黑色素瘤的早期检测对患者的生存至关重要。新出现的恶性肿瘤的一个识别特征是病变部位的血流增加。使用Nevoscope进行多光谱透照已被证明是一种用于对皮肤病变的皮下血管结构进行成像的有效工具。利用从该工具获得的可见光和近红外范围内的多光谱图像，以及由于氧合血红蛋白和脱氧血红蛋白导致的光谱吸收的相对差异，我们提出了一种经验方法来估计皮肤病变内的血流量。从这些图像中，计算出血红蛋白（Hb）和氧合血红蛋白（HbO₂）的分布估计值，以及一个描述血液中相对氧饱和度水平的比率特征。我们通过对嵌入毛细血管的皮肤模型进行成像来验证我们提出的方法，该模型可以填充人工Hb或HbO₂液体。我们的近红外多光谱计算能够很好地区分填充Hb的模型和填充HbO₂的模型，表明这些发色团可以成功分离并单独表征，用于估计皮肤组织的相对氧饱和度。
