Agostinho Marcelo, Américo Madileine F, Marques Rozemeire G, Zandoná Ednaldo Alexandre, Stelzer Murilo, Corá Luciana A, Andreis Uilian, Oliveira Ricardo B, Miranda José Ricardo A
Univ. Estadual Paulista-Unesp, Brazil.
Annu Int Conf IEEE Eng Med Biol Soc. 2010;2010:5740-3. doi: 10.1109/IEMBS.2010.5627855.
The aim of this study was to validate the alternate current biosusceptometry (ACB) for monitoring gastric contractions in rats. In vitro data were obtained to establish the relationship between ACB and the strain-gauge (SG) signal amplitude. In vivo experiments were performed on rats with magnetic markers and SGs previously implanted under the gastric serosa. The effects of the prandial state in gastric motility profiles were obtained. The correlation between in vitro signal amplitudes was strong (R = 0.989). The temporal cross-correlation between the ACB and SG signal amplitude was higher in the postprandial than in the fasting state. Irregular signal profiles, low contraction amplitudes, and smaller signal-to-noise ratios explained the poor correlation for fasting-state recordings. The contraction frequencies using ACB were 0.068 ± 0.007 Hz (postprandial) and 0.058 ± 0.007 Hz (fasting) and those using SG were 0.066 ± 0.006 Hz (postprandial) and 0.059 ± 0.008 Hz (fasting) (P < 0.003). When a magnetic tracer was ingested, there was a strong correlation and a small phase-difference between techniques. We conclude that ACB provides an accurate and sensitive technique for studies of GI motility in the rat.
本研究的目的是验证用于监测大鼠胃收缩的交变电流生物电阻抗测量法（ACB）。获取体外数据以建立ACB与应变片（SG）信号幅度之间的关系。对先前在胃浆膜下植入磁性标记物和SG的大鼠进行体内实验。获得了进食状态对胃动力曲线的影响。体外信号幅度之间的相关性很强（R = 0.989）。进食后ACB与SG信号幅度之间的时间互相关性高于禁食状态。不规则的信号曲线、低收缩幅度和较小的信噪比解释了禁食状态记录相关性较差的原因。使用ACB的收缩频率为0.068±0.007Hz（进食后）和0.058±0.007Hz（禁食），使用SG的收缩频率为0.066±0.006Hz（进食后）和0.059±0.008Hz（禁食）（P<0.003）。当摄入磁性示踪剂时，两种技术之间存在很强的相关性和很小的相位差。我们得出结论，ACB为大鼠胃肠动力研究提供了一种准确且灵敏的技术。
