Department of Biophysics and Pharmacology, São Paulo State University, Biosciences Institute of Botucatu, Botucatu, Brazil.
Science and Technology Institute, Federal University of São Paulo, São José dos Campos, Brazil.
Biomed Tech (Berl). 2021 Feb 3;66(4):405-412. doi: 10.1515/bmt-2020-0202. Print 2021 Aug 26.
The identification of gastrointestinal (GI) motility disorders requires the evaluation of regional GI transit, and the development of alternative methodologies in animals has a significant impact on translational approaches. Therefore, the purpose of this study was to validate an easy and low-cost methodology (alternate current biosusceptometry - ACB) for the assessment of regional GI transit in rats through images. Rats were fed a test meal containing magnetic tracer and phenol red, and GI segments (stomach, proximal, medial and distal small intestine, and cecum) were collected to assess tracer's retention at distinct times after ingestion (0, 60, 120, 240, and 360 min). Images were obtained by scanning the segments, and phenol red concentration was determined by the sample's absorbance. The temporal retention profile, geometric center, gastric emptying, and cecum arrival were evaluated. The correlation coefficient between methods was 0.802, and the temporal retention of each segment was successfully assessed. GI parameters yielded comparable results between methods, and ACB images presented advantages as the possibility to visualize intrasegmental tracer distribution and the automated scan of the segments. The imaging approach provided a reliable assessment of several parameters simultaneously and may serve as an accurate and sensitive approach for regional GI research in rats.
胃肠动力障碍的鉴定需要评估区域性胃肠道转运，而动物替代方法的发展对转化方法有重大影响。因此，本研究的目的是通过图像验证一种简单且低成本的方法（交流生物磁化率测定法 - ACB），以评估大鼠的区域性胃肠道转运。大鼠喂食含有磁性示踪剂和酚红的试验餐，收集胃肠道段（胃、近端、中间和远端小肠以及盲肠），以在摄入后不同时间（0、60、120、240 和 360 分钟）评估示踪剂的保留情况。通过扫描段获得图像，并通过样品的吸光度来确定酚红浓度。评估了时间保留曲线、几何中心、胃排空和盲肠到达情况。两种方法之间的相关系数为 0.802，并且成功评估了每个段的时间保留情况。两种方法的胃肠道参数均得到可比结果，而 ACB 图像具有可视化段内示踪剂分布和自动扫描段的优势。这种成像方法可同时可靠地评估多个参数，并且可以作为大鼠区域性胃肠道研究的准确和敏感方法。
