Sela D A, Chapman J, Adeuya A, Kim J H, Chen F, Whitehead T R, Lapidus A, Rokhsar D S, Lebrilla C B, German J B, Price N P, Richardson P M, Mills D A
Microbiology Graduate Group, Robert Mondavi Institute for Wine and Food Sciences, University of California, Davis, CA 95616, USA.
Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18964-9. doi: 10.1073/pnas.0809584105. Epub 2008 Nov 24.
Following birth, the breast-fed infant gastrointestinal tract is rapidly colonized by a microbial consortium often dominated by bifidobacteria. Accordingly, the complete genome sequence of Bifidobacterium longum subsp. infantis ATCC15697 reflects a competitive nutrient-utilization strategy targeting milk-borne molecules which lack a nutritive value to the neonate. Several chromosomal loci reflect potential adaptation to the infant host including a 43 kbp cluster encoding catabolic genes, extracellular solute binding proteins and permeases predicted to be active on milk oligosaccharides. An examination of in vivo metabolism has detected the hallmarks of milk oligosaccharide utilization via the central fermentative pathway using metabolomic and proteomic approaches. Finally, conservation of gene clusters in multiple isolates corroborates the genomic mechanism underlying milk utilization for this infant-associated phylotype.
出生后，母乳喂养的婴儿胃肠道会迅速被一个通常以双歧杆菌为主导的微生物群落定殖。因此，婴儿双歧杆菌亚种ATCC15697的完整基因组序列反映了一种针对对新生儿无营养价值的乳源分子的竞争性营养利用策略。几个染色体位点反映了对婴儿宿主的潜在适应性，包括一个43 kbp的簇，该簇编码预计对乳寡糖有活性的分解代谢基因、细胞外溶质结合蛋白和通透酶。通过代谢组学和蛋白质组学方法对体内代谢的研究已经检测到通过中心发酵途径利用乳寡糖的特征。最后，多个分离株中基因簇的保守性证实了这种与婴儿相关的系统型利用乳汁的基因组机制。
