Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, The Netherlands.
Biodegradation. 2012 Feb;23(1):117-25. doi: 10.1007/s10532-011-9491-z. Epub 2011 Jul 5.
Arthrobacter sp. strain G1 is able to grow on 4-fluorocinnamic acid (4-FCA) as sole carbon source. The organism converts 4-FCA into 4-fluorobenzoic acid (4-FBA) and utilizes the two-carbon side-chain for growth with some formation of 4-fluoroacetophenone as a dead-end side product. We also have isolated Ralstonia sp. strain H1, an organism that degrades 4-FBA. A consortium of strains G1 and H1 degraded 4-FCA with Monod kinetics during growth in batch and continuous cultures. Specific growth rates of strain G1 and specific degradation rates of 4-FCA were observed to follow substrate inhibition kinetics, which could be modeled using the kinetic models of Haldane-Andrew and Luong-Levenspiel. The mixed culture showed complete mineralization of 4-FCA with quantitative release of fluoride, both in batch and continuous cultures. Steady-state chemostat cultures that were exposed to shock loadings of substrate responded with rapid degradation and returned to steady-state in 10-15 h, indicating that the mixed culture provided a robust system for continuous 4-FCA degradation.
节杆菌属菌株 G1 能够以 4-氟肉桂酸（4-FCA）作为唯一碳源生长。该菌将 4-FCA 转化为 4-氟苯甲酸（4-FBA），并利用两碳侧链进行生长，同时形成一些 4-氟苯乙酮作为末端副产物。我们还分离到了能够降解 4-FBA 的罗尔斯通氏菌属菌株 H1。在分批和连续培养中，菌株 G1 和 H1 的混合菌降解 4-FCA 遵循 Monod 动力学。观察到菌株 G1 的比生长速率和 4-FCA 的比降解速率遵循底物抑制动力学，可以使用 Haldane-Andrew 和 Luong-Levenspiel 的动力学模型进行建模。混合培养在分批和连续培养中均能完全矿化 4-FCA，并定量释放氟化物。在稳态恒化器培养中，当受到底物冲击负荷时，混合培养能够快速降解，并在 10-15 h 内恢复到稳态，这表明混合培养为连续 4-FCA 降解提供了一个稳健的系统。
