基因多样的C组轮状病毒在韩国犊牛中引起散发性感染。
Genetically diverse group C rotaviruses cause sporadic infection in Korean calves.
作者信息
Park Sang-Ik, Jeong Young-Ju, Kim Hyun-Jeong, Park Jun-Gyu, Kang Shien-Young, Woo Sang-Kyu, Kim Chae-Hyeon, Jung Cheong-Hwan, Kang Mun-Il, Cho Kyoung-Oh
机构信息
Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500–757, South Korea.
出版信息
J Vet Med Sci. 2011 Apr;73(4):479-82. doi: 10.1292/jvms.10-0280. Epub 2010 Nov 19.
This study examined the prevalence and genetic diversity of the bovine group C rotaviruses (GCRVs) in a total of 127 diarrhea fecal samples of calves from 52 Korean native beef calf herds using RT-PCR and nested PCR. Overall, seven of the 127 fecal samples (5.5%) from seven of the 52 herds (13.5%) tested positive for bovine GCRVs only by nested PCR. Sequence and phylogenetic analyses of a partial VP6 gene showed that Korean bovine GCRVs had marked genetic diversity; two Korean strains belonged to the bovine lineage, whereas five Korean strains belonged to the porcine lineage. These results suggest that the genetically diverse bovine GCRVs cause sporadic infections in diarrheic calves in South Korea.
本研究采用逆转录聚合酶链反应（RT-PCR）和巢式聚合酶链反应，对来自52个韩国本土肉牛犊牛群的127份腹泻粪便样本中的牛C组轮状病毒（GCRV）的流行情况和遗传多样性进行了检测。总体而言，127份粪便样本中的7份（5.5%），来自52个牛群中的7个（13.5%），仅通过巢式PCR检测出牛GCRV呈阳性。对部分VP6基因的序列和系统发育分析表明，韩国牛GCRV具有显著的遗传多样性；两个韩国毒株属于牛谱系，而五个韩国毒株属于猪谱系。这些结果表明，遗传多样的牛GCRV在韩国腹泻犊牛中引起散发性感染。
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