Genotyping and determining the distribution of prevalent G and P types of group A bovine rotaviruses between 2010 and 2012 in Iran.

Determination and distribution of the G and P genotypes of group A bovine rotavirus was investigated on 386 fecal samples collected from calves with diarrhea using a semi-nested RT-PCR typing assay. Samples were collected from 11 provinces of Iran during 2010-2012. The provinces divided into 5 different groups based on geographical distance and climates. One hundred and nine samples (28.2%) were confirmed positive for rotavirus group A using ELISA. 75 positive samples were selected randomly and subjected to typing assay. G10 (50.6%) and P[11] (64%) were detected more than G6 (21.3%) and P[5] (9.3%). No any G8 and P[1] were observed. Of the 75 samples analyzed by RT-PCR in each geographical areas named as the south of Alborz mountain ranges area, the north-east area, the central area, the north-west area and the south-east area, number of samples with G10 genotype were 19, 9, 9, 0 and 1; G6 were 8, 0, 3, 5 and 0; P[11] were 25, 7, 11, 5 and 0 and finally P[5] were 5, 0, 2, 0 and 0 in each area, respectively. The most common VP7/VP4 combinations were G10P[11] (40%), G6P[11] (12%), G6P[5] (5.3%) and G10P[5] (2.6%). Phylogenetic analysis of one strain showed high identity with strain B223. Since the identification of G and P genotypes and their diversity is fundamental to development and use of effective vaccines, we determined the most prevalence G and P genotypes of bovine rotavirus group A (BRVA) in a broad area of Iran.